BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Genomic Heterogeneity in Clinical Strains of Mycobacterium tuberculosis, M. terrae Complex, M. gordonae, M. avium-intracellulae Complex and M. fortuitum by Pulsed-Field Gel Electrophoresis

  • Kim, Jeong-Ran (Department of Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University) ;
  • Kang, Bong-Seok (Department of Biochemistry and Molecular Biology, CoJlege of Oriental Medicine, Dongguk University) ;
  • Ko, Jeong-Heon (Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Jin-Suk (Department of Microbiology, Hannam University) ;
  • Kim, Sang-Jae (Korean Institute of Tuberculosis) ;
  • Bai, Gil-Hwan (Korean Institute of Tuberculosis) ;
  • Chung, Tae-Ho (Kyungpook National University School of Medicine) ;
  • Nam, Kyung-Soo (College of Medicine, Dongguk University) ;
  • Choi, Yong-Kyung (Korea Research Institute of Bioscience and Biotechnology) ;
  • Choe, In-Sung (Korea Research Institute of Bioscience and Biotechnology) ;
  • Chung, Tae-Wha (Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Young-Choon (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Cheorl-Ho (Department of Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University)
  • Received : 1996.07.24
  • Published : 1996.11.30
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Abstract

Clinical strains of Mycobacterium tuberculosis, M. terrae complex, M. gordonae, M. avium-intracellulae complex, and M. fortuitum from Korean patients were isolated and analyzed by comparing large restriction fragment (LRF) patterns produced by digestion of genomic DNA with infrequent-cutting endonucleases like AsnI and XbaI. and pulsed-field gel electrophoresis (PFGE). Three M. tuberculosis, two M. terrae complex, two M. gordonae, two M. avium-intracellulae complex, and two M. fortuitum strains were compared by using AsnI and XbaI. and this allowed easy visual separation of all epidemiologically unrelated strains. PFGE exhibits different DNA restriction patterns which are easy to compare. Genome size of the strains roughly ranged from 3020 to 3335 kb. The LRF patterns are useful for epidemiologic studies of tuberculosis with regard to drug resistance.

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References

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