한국균학회지 (The Korean Journal of Mycology)

한국균학회 (The Korean Society of Mycology)
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노루궁뎅이버섯(Hericium erinaceus)의 새로운 균사배양기의 조성

Composition of a New Medium for Mycelial Growth of Hericium erinaceus

  • 고한규 (고려대학교 자연자원대학 농생물학과) ;
  • 김동명 (대웅제약 중앙연구소) ;
  • 박원목 (고려대학교 생명공학원)
  • Ko, Han-Gyu (Department of Agricultural Biology, College of Natural Resources, Korea University) ;
  • Kim, Dong-Myong (Daewoong Pharmaceutical Co., Research and Development Center) ;
  • Park, Won-Mok (Graduate School of Biotechnology, Korea University)
  • 발행 : 1997.12.30
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초록

본 실험은 노루궁뎅이버섯(Hericium erinaceus isolate KU-1)의 균사생장에 관한 영양원 실험으로 대량의 균사체 수확을 위한 배양기 조성을 밝히기 위한 기초를 확립하고자 실시하였다. 최적온도는 $25^{\circ}C$이며 pH4에서 160.5 mg으로 가장 건물중이 높았고 탄소원 중 glucose, sucrose, galactose, mannose 순으로 균사생장 및 균사밀도가 우수하였다. 무기태질소원으로는 ammonium acetate 30 mM에서 균사생장이 가장 양호하였다. 아미노산 중 arginine 15 mM에서 우수하였다. C/N율 200에서 35.9 mm로 균사생장의 최대치를 보였다. 상술한 실험의 결과를 토대로 Table 1과 같은 새로운 합성배지를 조제할 수 있었고, 이를 Ko배지라 명명하였다. 이 Ko배지와 기존배지간의 한천배지상에서 균사생장 비교한 결과, Ko배지에 가장 우수한 균사생장과 균사밀도를 나타냈다. Ko배지의 조성은 glucose 18.02g, arginine 3.613g, ammonium acetate 2.313g, $CaCl_2\;0.33g$, $KH_2-PO_4\;8.5g$, $MgSO_4{\cdot}7H_2O\;2.0g$, $FeSO_4{\cdot}7H_2O\;0.02g$, $ZnSO_4{cdot}7H_2O\;0.02g$, $MnSO_4{\cdot}7H_2O\;0.02g$, water 1 liter이다.

These researches were carried out for improvement of medium for mycelial growth of Hericium erinaceus isolate KU-1. It grew well at pH 4 and $25^{\circ}C$. Glucose and sucrose were favorable carbon sources for mycelial growth. As nitrogen sources, ammonium acetate and arginine enhanced mycelial growth. Optimum C/N ratio was 200. Based On the results, the following recipe is suggested for synthetic medium for the mycelial growth: glucose 18.02 g, arginine 2.613 g, ammonium acetate 2.313 g, $CaCl_2\;0.33\;g$, $KH_2PO_4\;8.5\;g$, $MgSO_4{\cdot}7H_2O\;2.0\;g$, $FeSO_4{\cdot}7H_2O\;0.02\;g$, $ZnSO_4{cdot}7H_2O\;0.02\;g$, $MnSO_4{\cdot}7H_2O\;0.02\;g$, water 1 liter. This medium was superior for the mycelial growth to other conventional media such as Yeast malt extract agar (YMA), Park medium, Potato dextrose agar (PDA), Malt extract agar, Czapek-dox agar, Macaya-lizano medium and Yeast extract agar. This new synthetic medium is designated as Ko medium.

키워드

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