Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Isolation and Characterization of Benzoic Acid with Antimicrobial Activity from Needle of Pinus densiflora

솔잎에서 항미생물 활성을 갖는 benzoic acid의 분리 및 동정

  • Kuk, Ju-Hee (Department of Food Science and Technology, Chonnam National University) ;
  • Ma, Seung-Jin (Department of Food Science and Technology, Chonnam National University) ;
  • Park, Keun-Hyung (Department of Food Science and Technology, Chonnam National University)
  • Published : 1997.04.01
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Abstract

The ethyl acetate (EtOAc) extracts from needles of Pinus densiflora were showed antimicrobial activities against bacteria, yeast and fungi. The antimicrobial active substance of EtOAc extracts were successively purified with solvent fractionation, silica gel adsorption column chromatography and Sephadex LH-20 column chromatography. The purified active substance was isolated as crystals and identified as benzoic acid by $MS,\;^{1}H-NMR\;and\;^{13}C-NMR$. The amount of benzoic acid was 0.608 mg per gram of fresh needle of Pinus densiflora.

소나무(Pinus densiflora Siebold et Zuccarini) 잎의 ethyl acetate 추출물은 세균, 효모, 곰팡이에 대하여 항미생물활성을 나타내었다. 활성물질을 solvent fractionation, silica gel adsorption column chromatography, Sephadex LH-20 column chromatography 등에 의해 정제하여 백색결정상 물질로 단리하였다. $MS,\;^{1}H-NMR,\;^{13}C-NMR$ 등의 기기분석에 의해 단리된 물질의 구조해석을 시도한 결과 benzoic acid로 동정되었다. Benzoic acid가 솔잎에서 분리, 동정된 것은 처음으로 생각되며 특히, 솔잎 생체중량 g당 0.608 mg이나 함유되어 있어, 타 식물체에 비하여 대단히 많은 양이 함유되어 있음이 확인되었다.

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