Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Isolation and Characterization of Cinnamic Acid with Antimicrobial Activity from Needle of Pinus densiflora

솔잎에서 항미생물 활성을 갖는 Cinnamic Acid 의 분리 및 동정

  • Kuk, Ju-Hee (Department of Food Science and Technology, Chonnam National University) ;
  • Ma, Seung-Jin (Department of Food Science and Technology, Chonnam National University) ;
  • Park, Keun-Hyung (Department of Food Science and Technology, Chonnam National University)
  • Published : 1997.08.01
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Abstract

The EtOAc extracts from needles of Pinus densiflora showed antimicrobial activities against bacteria, yeast and fungi. The antimicrobial principle was successively purified by solvent fractionation, silica gel adsorption column chromatography and Sephadex LH-20 column chromatography. The active substance was further purified by HPLC using $C_{18}$ column. The active substance was identified as trans-cinnamic acid by MS, $^{1}H-NMR\;and\;^{13}C-NMR$. The amount of cinnamic acid was $9.27\;{\mu}g$ Per gram of fresh needle of Pinus densiflora.

소나무(Pinus densiflora Siebold et Zuccarini) 잎의 EtOAc 추출물이 세균, 효모, 곰팡이에 대하여 항미생물활성을 나타냈다. 활성물질은 solvent fractionation, silica gel adsorption column chromatography, Sephadex LH-20 column chromatography 등에 의해 정제되고 HPLC에 의해 분리되었다. 분리된 물질은 MS, $^{1}H-NMR,\;^{13}C-NMR$ 등의 기기분석에 의해 trans-cinnamic acid로 동정되었으며 이 물질은 솔잎 생체중량 g당 $9.27\;{\mu}g$정도 함유되어 있었다.

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