Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Fractionation of Anticarcinogenic Enzyme Inducer(s) from Roasted Perilla

볶은 들깨박으로부터 암예방효소계 활성성분의 분획

  • Hong, Eun-Young (Institute of Basic Sciences and Dept. of Food Science and Nutrition, Inje University) ;
  • Kang, Hee-Jung (Dept. of Food Science and Nutrition, Pusan National University) ;
  • Suh, Myung-Ja (Dept. of Food Science and Nutrition, Pusan National University) ;
  • Nam, Young-Jung (Korea Food Research Institute) ;
  • Kwon, Chong-Suk (Dept. of Food Science and Nutrition, Andong National University) ;
  • Kim, Jong-Sang (Institute of Basic Sciences and Dept. of Food Science and Nutrition, Inje University)
  • 홍은영 (인제대학교 식품영양학과 및 기초과학연구소) ;
  • 강희정 (부산대학교 식품영양학과) ;
  • 서명자 (부산대학교 식품영양학과) ;
  • 남영중 (한국식품개발연구원) ;
  • 권정숙 (안동대학교 식품영양학과) ;
  • 김정상 (인제대학교 식품영양학과 및 기초과학연구소)
  • Published : 1997.04.01
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Abstract

Elevation of the activities of phase 2 enzymes such as quinone reductase(QR) provides protection against several types of neoplasia. In this study, we performed partial purification of QR inducer(s) from roasted and defatted perilla meal by solvent fractionation and thin layer chromatography. Cellular QR induction was most notable in chloroform fraction of roasted perilla extract, compared with other solvent fractions. QR inducer(s) was partially purified by TLC, with 0.8 of $R_f$ value in n-butanol : n-propanol : 2N-ammonium hydroxide(10 : 60 : 30). AHH-inducing activity in TLC fractions isolated from methanol extracts of roasted perilla comigrated with QR-inducing fraction, suggesting that QR and AHH are induced by the same compound. TLC fractions shown strong QR-inducing activity also had a potent antioxidative activity, suggesting that cellular QR enzyme is induced by antioxidant(s) present in roasted perilla.

볶은 들깨박에 존재하는 항암효소계 유도물질을 분리하기 위해 용매분획과 preparative TLC를 실시하여 이들에 대한 암예방지표효소인 quinone reductase와 AHH 유도활성을 조사하였다. 볶은 들깨박의 메탄올 추출물을 용매분획하여 QR을 측정한 결과 chloroform층에서 가장 높은 활성이 나타났다. 들깨박 메탄올 추출물을 TLC로 분리한 분획가운데 QR과 AHH유도활성은 F1$(R_{f}=0.8)$에서 가장 높았으며, 항산화능은 F1$(R_{f}=0.8)$과 F2$(R_{f}=0.7)$에서 가장 강한 것으로 나타나 QR유도성분과 항산화성분이 동일성분일 가능성이 높은 것으로 사료 된다.

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