The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Effects of Juglandis Semen extraction on oxidant-induced cell injury in lung tissues

폐(肺) 조직(組織)에서 산화성(酸化性) 세포(細胞) 손상(損傷)에 대(對)한 호도(胡桃) 추출액(抽出液)의 효과(效果)

  • Lee, Woo-Heon (Dept. of Internal Medicine, College of Oriental Medicine, Dong Guk University) ;
  • Seo, Woon-Gyo (Dept. of Internal Medicine, College of Oriental Medicine, Dong Guk University) ;
  • Jeong, Ji-Cheon (Dept. of Internal Medicine, College of Oriental Medicine, Dong Guk University)
  • 이우헌 (동국대학교 한의과대학 내과학교실) ;
  • 서운교 (동국대학교 한의과대학 내과학교실) ;
  • 정지천 (동국대학교 한의과대학 내과학교실)
  • Published : 1997.04.30
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Abstract

This study was undertaken to determine Juglandis Semen extraction (JS) has a protective effect against the cell injury caused by oxidants, t-butylhydroperoxide (t-BHP) and $H_{2}O_2$ in rabbit lung slices. Cell injury was estimated by measuring tissue water content and peroxidation of membrane lipids was assessed by measuring malondialdehyde (MDA), an end-product of lipid peroxidation. t-BHP significantly increased water content in lung tissues over concentrations of 2-10 mM, and such effects were prevented by 5% JS. JS exerted the beneficial effect in a dose-dependent manner. $H_{2}O_2$ (100 mM) also increased water content in tissues, which was almost completely prevented by 5% JS. t-BHP induced lipid peroxidation in a dose-dependent fashion in lung tissues over concentrations of 0.5-10 mM. JS significantly reduced t-BHP induced lipid peroxidation and oxidant-independent endogenous lipid peroxidation, and such effects were dose-dependent at concentration of 0.5-10%. JS prevented $H_{2}O_2$ (100 mM)-dependent lipid peroxidation. These results suggest that JS prevents ceil injury induced by oxidants in the lung, and such effects may be attributed to inhibition of lipid peroxidation. The precise mechanisms remains to be explored.

Keywords

References

  1. FASEB J v.4 Role of oxygen free radicals in carcinogenesis and brain ischemia Floyd RA
  2. FASEB J v.9 Oxidative processes and antioxidative defense mechanisms in the aging brain Reiter RJ
  3. Acta Neurol Scand v.126 Oxidants and the central nervous system:some fundamental questions. Is oxidant damage relevant to Parkinson's disease, Alzheimer's disease, traumatic injury or stroke? Halliwell B
  4. J Lab Clin Med v.119 Free radicals, antioxidants, and human disease:Where are we now? Halliwell B;Gutteridge JMC;Cross CE
  5. Proc Soc Exp BioI Med v.209 Mechanisms of oxidant stress-induced acute tissue injury Jaeschke H
  6. J Clin Invest v.88 Oxygen-dependent lipid peroxidation during lung ischemia Fisher AB;Dodia C;Ran Z;Ayene I;Exkenhoff RG
  7. Am J Physiol v.265 $PO_2$- dependent hydroxyl radical production during ischemia-reperfusion lung injury Fisher PW;Huang Y-C T;Kennedy TP;Piantadosi CA
  8. J Physiol v.486 Ozone-induced oxygen radical release from bronchoalveolar lavage cells and airway hyper-responsiveness in dogs Stevens WH;Conlon PD;O'Byrne PM
  9. Arch Biochem Biophys v.321 Oxidative damage to extracellular fluids by ozone and possible protective effects of thiols Van del Vliet A;O'Neill CA;Eiserich JP;Cross CE:
  10. J Appl Physiol v.78 Ozone toxicity in the rat. I. Effect of changes in ambient temperature on extrapulmonary physiological parameters Watkinson WP;Wiester MJ;Highfill JW
  11. Lab Invest v.47 Biology of disease. Free radicals and tissue injury Freeman BA;Crapo JD
  12. Biochem Pharmacol v.49 Antioxidant characterization. Methology and mechanism Halliwell B
  13. 山東中醫學院學報 v.15 no.3 27種中藥及SOD對體外大白鼠賂均漿過氧化脂質生成的影響 舒守琴(外)
  14. 中西醫結合雜誌 v.11 no.8 四大懷藥延緩裏老作用的硏究 李獻平(外)
  15. 中國中西醫結合雜誌 v.12 no.1 還少丹膠囊抗裏老及治療腎陽虛臨床觀察 杜辛(外)
  16. 中國中醫雜誌 v.16 no.3 六味地黃湯及其配伍對過氧化脂質及脂褐質含量的影響 蔣莹(外)
  17. 原色臨床本草學 辛民敎
  18. 本草學 李尙仁
  19. 本草學 全國韓醫科大學本草學敎授(編)
  20. Anal Biochem v.86 Determination of malonaldehyde precusor in tissue by thiobarbituric acid test Uchiyama M;Mihara M
  21. Anal Biochem v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford MM
  22. Biochem. J. v.131 Milk xanthine oxidase type D(dehydrogenase) and type O(oxidase):Purification and interconversion and some properties Batteli, N. G.;Lorenzoni, E.;Stirpe, F.
  23. Free Radicals In Biology v.6 Antioxidants, aging and longevity Cutler, RG.
  24. Free radical in biology Involvement of radical reaction in aging and carcinogenesis in medicinal chemistry Pryor, WA
  25. 中西醫結合雜誌 v.10 no.8 腎虛, 衰老與自由基的關係以及補腎藥對自由基的影響 粱曉春(外)
  26. 中國中西醫結合雜誌 v.12 no.1 五子衍宗液延緩衰老的臨床觀察 王學美(外)
  27. 中醫雜誌 v.29 no.1 中國傳統延緩衰老藥物的現代硏究槪述 李春生
  28. 中醫雜誌 v.30 no.4 腎虛與超氧化物岐化酶關係初探 陳晏珍(外)
  29. 東醫病理學 文濬典(外)
  30. 中藥補腎壯陽大全 許士凱(外)
  31. 補腎長壽奇方妙述 桑樹榮(外)
  32. 東醫腎系內科學 杜鎬京
  33. 中藥臨床手冊 上海中醫學院(編)
  34. 中國本草圖錄 v.一 蕭培根(外)
  35. 中國藥物大全 中國藥物大全編委會
  36. 呼吸系病實用方 顧保君
  37. 大方藥合編 黃度淵
  38. Lab Invest v.62 Biology of disease. Mechanisms of cell injury by activated oxygen species Farber JL;Kyle ME;Coleman JB
  39. Angew Chem Int Ed. v.25 Biochemistry of oxidant stress Sies H