Parasites, Hosts and Diseases
- Volume 35 Issue 3
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- Pages.189-196
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- 1997
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- 2982-5164(pISSN)
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- 1738-0006(eISSN)
The role of nitric oxide as an effector of macrophage-mediated cytotoxicity against Trichomonas vaginalis
질편모충에 대한 대식세포의 세포독성에 있어서 NO의 역할
- Park, Geon-Chae (Department of Obstetrics and Gynecology, College of Medicine, Yonsei University) ;
- Ryu, Jae-Suk (Department of Parasitology, College of Medicine, Hanyang University) ;
- Min, Deuk-Yeong (Department of Parasitology, College of Medicine, Hanyang University)
- Published : 1997.09.01
Abstract
The purpose of this study is to determine whether nitric oxide is involved in the extracellular killing of Trichomoncs uasinalis by mouse (BALB/c) peritoneal macrophages and RAW264.7 cells activated with LPS or rIFN-γ and also to observe the effects of various chemicals which affect the production of reactive nitrogen intermediates (RNl) in the cytotoxicity against T. vnginnlis. The cytotoxicity was measured by counting the release of (3H)-thymidine from labelled protozoa and NOa was assayed by Griess reaction. Nemonomethyl-L-arginine (L-NMHA), Nenitro-L-arginine methyl ester (NAME) and arginase inhibited cytotoxicity to T. vaginnlis and nitrite production by activated mouse perioneal macrophagrs and RAW 264.7 cells. The addition of excess L-arginine competitively restored trichomonacidal activity of macrophages. Exogenous addition of FeSO4 inhibited cytotoxicity to T. vaginaLis and nitric products of macrophages. From above results, it is assumed that nitric oxide plays an important role in the host defense mechanism of macrophages against T ucfinalis.
활성화된 대식세포에서 생산되는 NO가 질편모충에 대해 세포독성이 있는지를 관찰하고자 질소 중간산물에 영향을 주는 약제를 첨가한 후 nitrite 생산 및 세포독성에 미치는 영향을 관찰하였다. 대식세포로는 마우스(BAkBlc) 복강 대식세포와 마우스 복강 내 종양세포인 RAW264.7 세포로 LPS(lipopolysaccharide)나
Keywords
- Nitric oxide;
- mouse peritoneal macrophage;
- RAW264.7 cell;
- Trichomonas vaginalis;
- cytotoxicity;
- nitrite production