Archives of Pharmacal Research
- Volume 20 Issue 1
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- Pages.1-6
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- 1997
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- 0253-6269(pISSN)
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- 1976-3786(eISSN)
Determination of Microviscosity and Location of 1,3-Di(1-pyrenyl) propane in Brain Membranes
- Kang, Jung-Sook (Departments of Oral Biochemisrty and Molecular, College of Dentisty and Research Institute for Oral Biotechnology) ;
- Kang, In-Goo (Departments of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan University) ;
- Yun, Il (Departments of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan University)
- Published : 1997.02.01
Abstract
We determined the microviscosity of synaptosomal plasma membrane vesicles (SPMV) isolated from bovine cerebral cortex and liposomes of total lipids (SPMTL) and phospholipids (SPMPL) extracted from SPMV. Changes in the microviscosity induced by the range and rate of lateral diffusion were measured by the intramolecular excimerization of 1, 3-di(1-pyrenyl)propane (Py-3-Py). The microviscosity values of the direct probe environment in SPMV, SPMTL and SPMPL were 38.17, 31.11 and 27.64 cP, respectively, at
Keywords
- Microviscosities;
- Activation energies;
- Fluorescent probe technique;
- Brain membranes;
- Probe location;
- Polarity parameter;
- Polarizability parameter