Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Protoplast fusion between Lentinula edodes and Coriolus versicolor

  • Kim, Chaekyun (Department of Microbial Chemistry, College of Pharmacy, Seoul National University) ;
  • Choi, Eung-Chil (Department of Microbial Chemistry, College of Pharmacy, Seoul National University) ;
  • Kim, Byong-Kak (Department of Microbial Chemistry, College of Pharmacy, Seoul National University)
  • Published : 1997.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

Protoplast fusion between isoleucine-, argihine- and thymidine-requiring auxotroph $(Ile^{-}, Arg^{-}, Thy^{-})$ of Lentinula edodes and arginine-requiring auxotroph $(Arg^-)$ of Coriolus versicolor has been achieved using 30% polyethylene glycol (M.W.4000) in 10 mM $CaCl_2$-glycine solution (pH 8.0). Fusion hybrids were selected in the 0.6 M sucrose supplemented minimal media on the basis of nutritional complementation with fusion frequency of $7.4{\times}10{-6}$ The hybrids included both parental and non-parental types in colony morphology, growth rate and isozyme patterns. We succeeded inter-order protoplast fusion between the auxotrophs of Lentinula edodes and Coriolus versicolor overcoming the natural barriers of incompatibility. We examined the characteristics of the hybrids and clarified the fusion rocess using electron microscopy.

Keywords

References

  1. Arch. Pharm. Res. v.17 Studies on protoplast fusion between Lentinula edodes and Ganoderma Iucidum Bok,J.W.;Choi,E.C.;Kim.B.K.
  2. Experientia v.29 Syringaldazine, an effective reagent for detecting laccase and peroxidase in fungi Harkin,J.M.;Obst ,J.R.
  3. Biochem. Biophys. Res. Comm. v.149 A biological response modefier, PSK, inhibits reverse transcriptase in vitro Hirose,K.;Hakozaki,M.Kakuchi,T.;Atsunaga,K.;Yoshikumi,C.;Takahashi,M.;Tochikura,T.S.;Yamamoto,N.
  4. J. Agric. Lab. v.8 Physiological and ecological studies on Lentinus edodes (Berk.) Sing. Ishikawa,H.
  5. J. Gen. Microbiol v.102 Interspecific hybridization between Aspergillus nidulans and Aspergillus rugulosus by fusion of somatic protoplasts Kevei,F.;Peberdy,J.F.
  6. Mol. Gen. Genet. v.170 Induced segregation in interspecific hybrids of Aspergillus nidulans and Aspergillus rugulosus obtained by protoplast fusion Kevei,F.;Peberdy,J.F.
  7. Fungal protoplasts Interspecies hybridization after protoplast fusion in Aspergillus Kevei,F.;Peberdy,J.F.;Peberdy,J.F.(ed.);Ferenczy,L.(ed.)
  8. Arch. Pharm. Res. v.2 Studies on the constituents of higher fungi of Korea (XXIII), antineoplastic activities of Coriolus versicolor (L. exFr.) Quel., Pleurotus ostreatus (Fr.) Kummer and Lentinus edodes (Berk) Sing. Kim,B.K.;Park,E.K.;Shim,M.J.
  9. Korean J. Mycol. v.24 Isolation of auxotrophs and drug resistant mutants of Lentinus edodes Kim,C.;Shim,M.J.;Choi,E.C.;Kim,B.K.
  10. Arch. Pharm. Res. v.20 Liquid culture enhances isolation of protoplasts from the auxotroph (Ser-) of Lentinula edodes Kim,C.;Kim,B.K.
  11. Korean. J. Mycol. v.15 Studies on the artificial cultivation of Lentinus edodes on sawdust media Kim,H.K.;Park,Y.H.;Cha,D.Y.;Chung,H.C.
  12. Developmental Biology of Higher Fungi Growth and development of Lentinus edodes on chemically defined medium Leatham,G.F.;Moore,D.(ed.)
  13. Mushroom Sci. v.12 A study on intergeneric hybridization between Pleurotus sajor-caju and Schizophyllum commune by protoplast fusion Liang,Z.R.;Chang,S.T.
  14. Nature v.229 Lentinan, a new immuno-accelerator of cell mediated responces Maeda,Y.Y.;Chihara G.
  15. Gann v.66 Restriction of antibody-forming capacities by PS-K in tumor-bearing mice Nomoto,K.;Yoshikumi,C.;Matsunaga,K.;Fujii,T.;Takeya,K.
  16. Gann v.66 Antitumor effects of protein-bound polysaccharide preparation, PS-K, against 3-methyl cholanthrene-induced fibrosarcoma in C57BL/6 mice Ohno,R.;Imai,K.;Yokomaku,S.;Yamada,K.
  17. Trans. Mycol. Japan v.27 Analysis of esterase and malate dehydrgenase isozymes of Lentinus edodes by isoelectric focusing for the identification and discrimination of stocks. Ohmasa,M.;Furukawa,H.
  18. Proc. Int. Sym. on New Drug Development from Natural Products Studies on protoplast and nuclear fusion of Ganoderma species Park,S.H.;Choi,E.C.;Kim,B.K.
  19. Arch. Pham. Res. v.14 Studies on intergeneric protoplast fusion and neclear transfer between Ganoderma Iucidum and Coriolus versicolor Park,S.H.;Choi,E.C.;Kim,B.K.
  20. Korean J. Mycol. v.17 Comparisons of physiological characteristics in Coriolus versicolor intraspecific strains Park,Y.D.;Whang,W.K.;Huh,J.D.;Kim,S.H.;Park,W.M.
  21. Biochem. Biophys. Res. Comm. v.160 Inhibition of the infectivity and cytopathic effect of human immunodeficiency virus by water-soluble lignin in an extract of the culture medium of Lentinus edodes mycelia (LEM) Suzuki,H.;Okubo,A.;Yamazaki,S.;Suzuki,K.;Mitsuya,H.;Toda,S.
  22. Biochem. Genet. v.17 Linkage, chromosomal association, and expression of Adh-1 and Pgm-2 in tomato Tanksley,S.D.
  23. Biochem. Biophys. Res. Comm. v.148 A biological response modifier, PS-K, inhibits human immunodeficiency virus infecrion in vitro Tochikuro,T.S.;Nakashima,H.;Hirose,K.;Yamamoto,N.
  24. Mushroom Sci. v.11 On the application of isozyme electrophoresis to identification of strains in Lentinus edodes (Shiitake) Toyomasu,T.;Zennyozi
  25. Gann v.65 Protein-bound polysac-charide preparation, PS-K, effective against mouse sarcoma-180 and rat ascites hepatoma AH-13 by oral use Tsukagoshi,S.;Ohashi,F.
  26. Korean J. Mycol v.24 intergeneric transfer of isolated nuclei from Lentinus edodes into protoplasts of Pleurotus florida Yoo,Y.B.;Shin,P.G.