Archives of Pharmacal Research

  • 제20권6호
  • /
  • Pages.519-524
  • /
  • 1997
  • /
  • 0253-6269(pISSN)
  • /
  • 1976-3786(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

Heterogeneous Natures of the Microbial Steroid $9{\alpha}$-Hydroxylase in Nocardioforms

  • 발행 : 1997.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Steroid $9{\alpha}$-hydroxylase is an enzyme found in nocardioform microorganisms which can utilize steroids as a sole carbon source. After fractional centrifugation of the cell homogenates, the enzyme activity in Nocardia and Rhodococcus was found in cytoplasmic membrane fraction. On the contrary, Mycobacterium had its 9.alpha.-hydroxylation activity in cytosolic fraction. To characterize the enzyme in these microorganisms, several potential inhibitors of 9.alpha.-hydroxylase were tested and the cofactor requirement for the same enzyme was also examined. The inhibitory effect of ferrous ion chelators indicated involvement of iron containing proteins in the 9.alpha.-hydroxylase system. On the other hand, metyrapone, an inhibitor known to be specific for cytochrome P450 interfered with the enzyme in Mycobacterium, but didn't inhibit the enzyme activity in Nocardia and Rhodococcus. While the $9{\alpha}$-hydroxylase system in Nocardia and Rhodococcus required NADPH, NADH was required as an election donor in Mycobacterium.

키워드

참고문헌

  1. Appl. Microbiol. Biotechnol. v.19 Inducibility of cytochrome P450 in Acinetobacter calcoaceticus by n-alkanes Asperger,O.;Naumann,A.;Kleber,H.P.
  2. J. Biol. Chem. v.251 Characterization of a cytochrome P450-dependent steroid hydoxylase system present in Bacillus megaterium Berg,A.;Gustafsson,J.A.;Ingelman-Sundberg,M.
  3. J. Biol. Chem. v.254 Purification and characterization of cytochrome $P450_{meg}$ Berg,A.;Ingelman-Sundberg,M.;Gustaffson,J.A.
  4. Eur. J. Biochem. v.57 A 4-methoxybenzoate o-demethylase from Pseudomonas putida, a new type of monooxygenase system Bernhardt,F.H.;Pachowsky,H.;Staudinger,H.
  5. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford,M.
  6. Anal. Biochem. v.162 Adaptation of the Bradford protein assay to membrane-bound proteins by slubilizing in glucopyranoside detergents Bradford,O.F.
  7. Biochem. v.3 Mechanisms of steroid oxidation by microorganisms. Ⅶ. Properties of the 9α-hydroxylase Chang,F.N.;Sih,C.J.
  8. J. Bacteriol. v.129 p-Cymene pathway in Pseudomonas putida: initial reactions De Frank, J. J.;Ribbons,D.W.
  9. J. Am. Chem. Soc. v.83 Microbiological transformation: Ⅶ The hydroxylation of steroids at C-9 Dodson,R.M.;Muir,R.D.
  10. J. Am. Chem. Soc. v.110 Structural characterization by EXAFS spectroscopy of the binuclear iron center in Protein A of methane monooxygenase form Methylococcus capsulatus (Bath) Ericson,A.;Hedman,B.;Hodgson,K.O.;Green,J.;Dalton,H.;Bentsen,J.G.;Beer,R.H.;Lippard,S.J.
  11. J. Biol. Chem. v.266 Complex formation between the protein components of methane monookygenase from Methylosinus trichosporium OB 3b Fox,B.G.;Liu,Y.;Dege,J.E.;Lipscomb,J.D.
  12. J. Bacteriol. v.174 Purification and properties of the NADH reductase component alkene monooxygenase from Mycobacterium strain E3 Frans,J.W.;Willen,J.H.;Van Berkel;Hartmans,S.;Jan, A. M. De Bont.
  13. J. Gen. Microbiol. v.137 Alkene monooxygenase from Mycobacterium: a multicomponent enzyme Hartmans,S.;Frans,J.W.;Somhorst,D.P.M.;Jan A. M. De Bont.
  14. Biochims. Biophys. Acta v.431 Involvement of a signal hydroxylase species in the hydoxylation of palmitate at the ω-1, ω-2, and ω-3 positions by a preparation from Bacillus megaterium Ho,P.P.;Fulco,A.J.
  15. J. Biochem. v.99 Studies on steroid monooxygenase from Cylindrocarpon radicicola ATCC 11011; Purification and characterization Itagaki,E.
  16. J. Biol. Chem. v.243 A soluble cytochrome P450 fuctional role in methylene hydroxylation Katagiri,M.;Ganguli,B.N.;Gunsalus,I.C.
  17. Arch. Microbiol. v.149 Identification of constitutive and herbicide inducible cytochromes P450 in Streptomyces griseolus O'Keffe,D.P.;Romesser,J.A.;Leto,K.J.
  18. J. Biol. Chem. v.241 Fatty acid ω-hydroxylating system of Pseudomonas oleovorans Peterson,J.A.;Basu,D.;Coon,M.J.
  19. J. Gen. Microbiol. v.131 The effect of copper ions on membrane content and methane monooxygenase aecivity in methanol-grown cells of Methylococcus capsulatus (Bath) Prior,S.D.;Dalton,H.
  20. Abstr. 90th Ann. Melt. Am. Soc. Microbiol. Abstr. v.-54 Identification of a ferredoxin reductase in cell-free extracts of Streptomyces griseus Ramachandra,M.;Sariaslani,F.S.
  21. Microbial technology(2nd Ed.) v.1 Microbiol Transformation of Steroids and Stereols Sebek,O.K.;Perlman,D.;Peppler,H.J.(ed.);Perlman,D.(ed.)
  22. Biotech. Lett. v.5 Copper stress underlies the fundamental change in intracellular location of methane monooxygenase in methane-oxydizing organisms: Studies in batch and continues cultures Stanley,S.H.;Prior,S.D.;Leak,D.J.;Dalton,H.
  23. Eur. J. Biochem. v.128 The steroid 9α-hydroxylation system from Nocardia species Strijewski,A.
  24. J. Bacteriol. v.171 Purification and characterization of a soybean flour-in-duced cytochrome P450 from Streptomyces griseus Trower,M.K.;Sariaslani,F.S.;O'keefe,P.O.
  25. J. Biol. Chem. v.265 Protein components of a cytochrome P450 linalool 8-methyl hydroxylase Ullah,A.J.H.;Murray,R.I.;Bhattacharyya,P.K.;Wagner,G.C.;Gunsalus,I.C.