Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Antihepatotoxic zeaxanthins from the Fruits of Lycium chinense

  • Published : 1997.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

A $CHCl_3$:MeOH extract of the fruit of Lycium chinense Mill. (Solanaceae) was found to afford significant protection against carbon tetrachloride-induced toxicity in primary cultures of rat hepatocytes. Subsequent activity-guided fractionation resulted in the isolation of zeaxanthin and zeaxanthin dipalmitate as antihepatotoxic components. Incubation of injured hepatocytes with zeaxanthin dipalmitate reduced the levels of glutamic pyruvic transaminase (GPT) and sorbitol dehydrogenase (SDH) released from damaged cells to 60.5% and 76.3% of those released from untreated controls, respectively. Zeaxanthin also reduced the levels of GPT and SDH to 68.5% and 61.3% of the levels of those released from the untreated contro. The results confirm the hepatoprotective activities of zeaxanthins. Antihepatotoxic activities of zeaxanthins are comparable to that of silybin.

Keywords

References

  1. J. Chem. Soc. C. Optical ratatory dispersion of carotenoids Bartlett,L.;Klyne,W.;Mose,W.P.;Scopes,P.M.;Galasco,G.;Mallams,A.K.;Weedon,B.C.L.;Szabolcs,J.;Toth,G.
  2. J. Cell. Biol. v.43 High yield preparation of isolated rat liver parenchymal cells Berry,M.N.;Friend,D.S.
  3. Laboratory Techniques in Biochemistry and Molecular Biology v.21 High-yield preparation of isolated hepatocytes from rat liver Berry,M.N.;Edward,A.M.;Barrit,G.J.;Burdon,R.H.(ed.);Knippenberg,P.H.(ed.)
  4. J. Chem. Soc. C. Carotenoids and related compounds, Part XX, Structure and reactions of fucoxanthin Bonnet,R.;Mallams,A.K.;Spark,A.A.;Tee,J.L.;Weedon,B.C.L.;McCormick,A.
  5. Rev. Latinoam. Quim. v.15 Fisalieno, colorante de los frutus maduros de plants Clccio,J.F.;Castro,V.H.
  6. Methods of Enzymatic Analysis Sorbitol dehydrogenase Gerlach,U.;Bergmeyer,H.U.(ed.)
  7. Plnat Pigments Distribution and Analysis of Carotenoids Goowin,T.W.;Britton,G.;Goowin,T.W.(ed.)
  8. Phytochem. v.17 Four new and other 4-methylsterols in the seeds of Solanaceae Itoh,T.;Tamura,T.;Matsomoto,T.
  9. J. Nat. Prod. v.60 New antihepatotoxic cerebroside from Lycium chinense fruits Kim,S.Y.;Choi,Y.;Huh,H.;Kim,J.;Kim,Y.C.;Lee,H.S.
  10. Planta Med. v.49 Assay method for antihepatotoxic activity using carbon tetrachloride induced cytotoxicity in primary cultured hepatocytes Kiso,Y.;Tohkin,M.;Hikino,H.
  11. Shikoku Igaku Zasshi v.18 General pharmacological studies of the water and various organic solvent soluble components derived from Lycium chinese Kukokawa,S.
  12. Planta Medica v.61 Antihepatotoxic activity of icariin, a major constituent of Epimedium koreanum Lee,M.K.;Choi,Y.J.;Sung,S.H.;Shin,D.I.;Kim,J.;Kim,Y.C.
  13. Rev. Latinoam. Quim. v.15 Alcaloides en dos especifs de Lycium Maldoni,B.E.
  14. Pure & Appl. Chem. v.47 Carbon-¹³NMR spectra of carotenoids Moss,G.P.
  15. Nippon Shokuhin Kogyo Gakkaishi v.10 Betaine of Lycium chinense Nishiyama,R.
  16. Am. J. Clin. Pathol. v.28 A colormetric method for the determination of glutamic oxaloacetic and glutamic pyruvic transaminase Reitman,S.;Frankel,S.
  17. Agric. Biol. Chem. v.47 Natural voletile components of "Kukoshi" (Lycium chinese M.) Sannai,A.;Fujimori,T.;Kato,K.