Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Effects of Acidification on the Species Compositions of Heterotrophic Bacterial Community in Microcosm

수계 종속영양세균 군집의 종조성에 미치는 산성화의 영향

  • 안영범 (한양대학교 생물학과) ;
  • 조홍범 (서울대학교 분자생물학 연구센터) ;
  • 최영길 (서경대학교 생물공학과)
  • Published : 1997.09.01
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Abstract

In an artificial pH-gradient hatch culture system, the author analyzed the effects of acidification on the species composition of heterotrophic bacteria. As the result of this study, it was found that the numbers of total bacteria were not affected by acidification and that the population size of heterotrophic bacteria decreased as pH became lower. The heterotrophic bacteria isolated from all of the pH gradient were 12 genera and 22 species. and among them, gram negative and gram positive bacteria were 64% and 36%, respectivcly. As pH decreased, the distribution rate of gram negative bacteria increased while that of gram positive bacteria decreased. Regarding to distrihution rate of genuses in each pH gradient, 13 genuses appeared at pH 7 while only 5 genuses appeared at pH 3. which means that the diversity of genera decrease as pH decreased. As a result of cluster analysis, diversity indices 01 species had ranges from 1.13 to 2.37, and decreased as pH decreased. In order to evaluate the diversity of different size samples, we analyzed the expected number of species appearance according to pH by rarefaction method. The statistical significance of species diversity was verified by the fact that the number decreased at lower pH.

인위적으로 pH 구배가 조성딘 회분배양 시스템(batch culture system)내에서 종속영양 세균 군집의 종조성 변화에 미치는 산성화의 영향을 수리학적으로 분석하였다. 총세균의 개체수는 산성화에 영향을 받지 않았으며, 종속영양 세균 군집의 크기는 pH가 낮아질수록 감소하였다. 전 pH 구간에서 분리된 종속영양 세균들은 모두 12속 22종으로 나타났으며, 이중 그람음성 세균은 64%, 그람양성 세균은 36%의 비율로 분포하였다. pH가 낮아짐에 따라 그람양성 세균의 분포 비율은 감소하는 반면, 그람음성 세균의 분포 비율은 증가하는 양상을 보였다. 각 pH 구간별 속의 분포 비율은 pH 7에서는 12개 속이 출현하였으나, pH 3에서는 5개 속만 출현하여 pH가 낮아짐에 따라 속의 다양성이 감소하는 양상을 나타내었다. 종 다양성 지수는 1.13-2.37이 범주였으며, pH가 낮아질수록 종 다양성 지수가 낮게 나타났다. 크기가 다른 군집의 다양성 평가를 위해 rarefraction 방법으로 pH에 따른 종 출현의 기대값을 분석한 결과 역시 pH가 낮아질수록 기대값(expected number)이 낮은 것으로 나타남으로써 종 다양성 지수에 대한 유의성을 검정하였다.

Keywords

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