Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Bacillus stearothermophilus Acetylxylan Esterase 유전자(estI)의 염기 서열 결정

  • 이정숙 (고려대학교 생명공학원) ;
  • 최용진 (고려대학교 생명공학원)
  • Published : 1997.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

The nucleotide sequence of the estI gene encoding acetylxylan esterase I of Bacillus stearothermophilus was determined and analyzed. The estI gene was found to consist of a 810 base pair open reading frame coding for a polypeptide of 270 amino acids with a deduced molecular weight of 30 kDa. This was in well agreement with the molecular weight (29 kDa) estimated by SDS-PAGE of the purified esterase. The coding sequence was preceded by a putative ribo some binding site 10 bp upsteam of the ATG codon. Further 53 bp upstream, the transcription initiation signals were identified. The putative $_{-}$10 sequence (TCCAAT) and $_{-}$35 seqence (TTGAAT) corresponded closely to the respective consensus sequences for the Bacillus subtiis major RNA polymerase. The G+C content of the coding region of the estI was 51% whereas that of the third position of codone was 60.2%. The N-terminal amino acid sequence of the EstI deduced from the nucleotide sequence perfectly matched the corresponding region of the purified esterase described previously. Comparison with the amino acid sequence of other esterases and lipases reported so far allowed us to identify a sequence, GLSMG at positions 123 to 127 of the EstI which was reported to be the highly conserved active site sequence for those enzymes. The nucleotide sequence of the estI revealed 55.7% homology to that of the xylC coding for the acetylxylan esterase of Caldocellum saccharolyticum.

Keywords

References

  1. Appl. Environ. Microbiol. v.59 Regulation and Characterization of Xylanolytic Enzymes of Thermoanaerobacterium saccharolyticum B6ARI Lee, Yong-Eok;Lowe, Sue E.;Jeikus, J. Gregory
  2. Appl. Environ. Microbiol. v.56 Purification and Properties of an Acetyxylan Esterase from Fibrobacter succinogenes S85 McDermid, Kevin P.;Forsberg, Cecil W.;MacKenzie, C. Roger
  3. Appl. Microbiol. Biotechnol. v.28 An Acetyl Esterase of Trichoderma reesei and its role in the Hydrolysis of Acetyl Xylans Poutaner, K.;Sunberg, M.
  4. Appl. Biotech. v.20;21 The Role of Ester Groups in Resistance of Plant Cell Wall Polysaccharides to Enzymatic Hydrolysis Gormann, K.;Mitchell, D. J.;Himmel, M. E.;Dale, B. E.;Schroeder, H. A.
  5. Kor. J. Appl. Microbiol. Biotech. v.23 no.4 Purification and characterization of Acetylxylan Esterase II from E. coli Cells Harboring the Recombinant Plasmid pKMG6 Kim, H. S.;Suh, J. H.;Choi, Y. J.
  6. Kor. J. Appl. Microbiol. Biotech. v.21 Molecular Cloning and Expression of the Acetylxylan Esterase Gene of Bacillus stearothermophilus in Escherichia coli Kim, H. S.;Cho, S. G.;Choi, Y. J.
  7. Molecular Cloning, a Laboratory Manual Maniatis, T.;Fritsch, E. F.;Sambrook, J.
  8. DNA Cloning v.I Techniques for Transformation of E. coli Hanahan, D.;D. M. Glover(ed.)
  9. Mol. Gen. Genet. v.186 Nucleotide Sequences that signal the Initiation of Transcription and Translation in Bacillus subtilis Moran Jr. C. P.;Lang, N.;LeGrice, S. F. J.;Lee, G.;Stephens, M. A.;Sonenshein, L.;Pero, J.;Losick, R.
  10. Kor. J. Appl. Microbiol. Biotech. v.22 Sequences Analysis of β-xylosidase Gene from Bacillus stearothermophilus Oh, H. J.;Choi, Y. J.
  11. Kor. J. Appl. Microbiol. Biotech. v.20 no.3 Molecular Cloning and Expression of an Endo-xylanase from Bacillus stearothermophilus into Escherichia coli Cho, S. G.;Park, S. S.;Park, Y. I.;Choi, Y. J.
  12. Ann. Rev. Biochem. v.57 Posttranscriptional Regulatory mechanisms in Escherichia coli Sarry Gold
  13. J. Bacteriol. v.163 Nucleotide Sequence and Promoter Region for the Neutral Protease Gene from Bacillus stearothermophilus Takagi, M.;T. Imanaka;Aiba, S.
  14. J. Biol. Chem. v.259 High Guanine Plus Cytosine Content in the Third Letter of Codons of an Extreme Thermophile Nagaqa, Y.;Nojima, H.;Isgizuka, K.;Makajima, R.;Yasuhara, R.;Ranaka, R.;Oishima, R.
  15. J. Bacteriol. v.163 Nulceotide Sequence of Bacillus stearothermophilus α-amylase Gene Nakajima, T.;R. Imanaka;Aiba, S.
  16. Eur. J. Biochem. v.132 The Amino Acid Sequence of the Tyrosyl-tRNA Synthetase from Bacillus stearothermophilus Winter, G.;Koch, G. L. E.;Hartley, B.;Barker, D.
  17. J. Bacteriol. v.160 Cloning of the Neutral Protease Gene of Bacillus subtilis and the Use of the Cloned Gene to Create an In Vitro-derived Deletion Mutation Yang, M. Y.;Ferrari, E.;Henner, D. J.
  18. J. Bacteriol. v.158 Replacement of the Bacillus subtilis Subtilisin Structural Gene with an In Vitro-derived Deletion Mutation Stahl, M. L.;Ferrari, E.
  19. J. Bacteriol. v.159 Genes for Alkaline Protease and Neutral Protease from Bacillus amyloliquefaciens Contain an Large Open Reading Frame between the Rigions Coding for Signal Sequence and Mature Protein Vasantha, M. L.;Thomson, D.;Rhodes, C.;Banner, C.;Nagle, J.;Filopula, D.
  20. Gene v.18 Preferential Codon Usage in Prokaryotic Genes: the Optimal Codon-anticodon Interaction Energy and the Selective Usage in Efficiently Expressed Genes Grosjean, H.;Fibers, W.
  21. Nucleic Acids Res. v.12 Evidence for Coding Pattern on the Noncoding Strand of the Escherichia coli Genome Allf-Steinberger, C.
  22. Nature v.334 The Molecular Evolution of Genes and Proteins: a Tale of Two Serines Sydney;Brenner
  23. Appl. Environ. Microbiol. v.56 Cloning, Sequence Analysis, and Expression of Genes Encoding Xylan-Degrading Enzymes from the Thermophile Caldocellum saccharolyticum Luethi, E.;Love, D. R.;McAnulty, J.;Wallace, C.;Caughey, P. A.;Saul, D.;Bergguist, P. L.