한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제25권2호
  • /
  • Pages.115-120
  • /
  • 1997
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

감귤류 변패의 원인균인 Penicillium sp.-L4가 생성하는 식물세포벽 분해효소의 작용양상

  • 김무성 ((주)태평양 기술연구원 기반기술연구소 Tel.82-331-281-8340 E-mail:prdcspch＠interpia.net) ;
  • 최영길 (한양대학교 생물학과)
  • 발행 : 1997.04.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Penicillium sp.-L4, a causative fungus of rot in citrus fruits, was isolated and its mode of hydrolytic enzyme production was investigated. Carboxymethylcellulase (CMCase), polygalacturonase(PGase), extra- & intra-cellular $\beta$-glucosidase and cellobiase were produced drastically by addition of substrates in minimal media. Production of the hydrolytic enzymes were induced efficiently by cellobiose and cellooligosaccharides which were the products of cellulose hydrolysis, but repressed by addition of mono-saccharide such as glucose, raffinose, galacturonic acid. The relative activity of p-nitrophenyl-$\beta$-D-glucopyranoside(PNPG) hydrolysis was higher than that of cellobiose hydrolysis in extracellular enzymes, and reverse is true in intracellular enzymes. Intact enzyme production of P. sp.-L4 on lemon peel lesion was sequential. $\beta$-Glucosidase and CMCase were produced first and followed by PGase. The enzyme productivities and pH in lesions were coincident with optimal pH of each enzyme activities.

키워드

참고문헌

  1. Introduction to plant biochemistry(2nd ed.) The plant cell wall Goodwin, T. W.;E. I. Mercer
  2. Trends Biotechnol. v.13 Notes from the underground: highlights from plant-microbe interactions Baron, C.;P. C. Zambryski
  3. 防菌防微 v.24 植物病原絲狀菌による宿主植物の認識と感染 山口勇;植草秀敏
  4. Physiol. Mol. Plant Pathol. v.47 Purification and structural determination of a phytotoxic substance from Exerohilum turcicum Bashan, B;R. S. Levy;M. Cojocaru;Y. Levy
  5. Can. J. Microbiol. v.42 Oxalate production by fungi: its role in pathogenicity and ecology in soil environment Dutton, M. V.;C. S. Evans
  6. Physiol. Mol. Plant Pathol. v.47 Involvement of protein phosphorylation in the induction of approessorium formation in Collectrichum gloeosporioides by its host surface wax and ethylene Flashiman, M. A.;C.-S. Hwang;P. E. Kolattukudy
  7. Physiol. Mol. Plant. Pathol. v.48 Chemical signals responsilble for appressorium formation in the rice blast fungus Magnaporthe grisea Gilbert, R. D.;A. M. Johnson;R. A. Dean
  8. Fundamentals of the fungi(2nd ed.) Fungi as parasites of plants Landecker, E. M.
  9. Mycol. Res. v.98 Fungal enzymes degrading plant cell walls: their possible significance in the ectomycorrhizal symbiosis Cairney, J. W. G;R. M. Burke
  10. Biotechnol. Bioeng. v.26 Hydrolysis of lignocellulose by Penicillium funiculosum cellulase Mishra, C.;M. Rao;R. Seeta;M. C. Srinivasan;V. Deshpande
  11. Mycol. Res. v.99 Comparison of xylanase production by fungal pathogens of barly with reference to Bipolaris sorokiniana Peltonen, S.
  12. Plant Physiol. v.51 Host-pathogen interactions Fisher, M. L.;A. J. Anderson;P. Albersheim
  13. Plant Physiol. v.60 Characteristics of tomato cell wall degradation in vitro Wallner, S. J.;H. L. Bloom
  14. Can. J. Microbiol. v.39 Purification and some properties of β-glucosidase from ectomycorrhizal fungus Pisolithus tinctorius strain Cao, W.;D. L. Grawford
  15. Biosci. Biotech. Biochem. v.60 β-Glucosidase from Botrytis cinerea: Its involvement in the pathgenicity of this fungus Sasaki, I.;H. Nagayama
  16. Mycol. Res. v.99 Invertase activity in Epichloe/Acremonium fungal endophytes and its possible role in choke disease Lam, C. K.;F. C. Belanger;J. F. White Jr.;J. Daie
  17. Food Technol. v.48 Citrus limonoid reduction of chemically induced tumorigenesis Lam, L. K. T.;J. Zhang;S. Hasegawa
  18. Edible fruits and nuts(ed.) Plant resources of southeast asia Verheij, E. W.;R. E. Coronel
  19. Introduction of food-born fungi(ed.) Samson, R. A.;E. S. Hoekstra;C. A. N. Van Oorschot
  20. 防菌防微 v.19 眞菌の分離と分類, 同定-Penicillium屬 宇田川俊一
  21. Anal. Biochem. v.28 Use of dinitrosalicylic acid reagent for determination of reducing sugars Miller, G. L.
  22. Phytopathology v.60 Induction of electrolyte loss, tissue maceration, and cellular death of potato tissue by an endopoly-galacturonate trans-eliminase Mount, M. S.;D. F. Bateman;H. G. Basham
  23. Can. J. Microbiol. v.21 Sequential production of polygalacturonase, cellulase, and pectin lyase by Rhizoctonia solani Lishker, N.;J. Katan;Y. Henis
  24. Phytopathology v.54 Cellulase and the Rhizoctonia disease of bean Bateman, D. F.
  25. 한국균학회지 v.23 잿빛곰팡이 병원균 Botrytis cinerea 균주의 분리기 주별 표현형적 특성, 병원성 및 약제저항성 변이 최인실;정영륜;조광연
  26. Agric. Biol. Chem. v.52 Purification and properties of two β-glucosidase from Penicillum herquei Banier and Sartory Funaguma, T.;A. Hara
  27. Enzymer Microb. Technol. v.12 β-Glucosidase production by the mycelial culture of the mushroom Termitomyces clypeatus Sengupta, S.;S. Sengupta
  28. Enzyme Microb. Technol. v.4 Fungal and other β-D-glucosidase; their properties and applications Woodward, J.;A. Wiseman
  29. Enzyme Microb. Technol. v.6 Kinetic and mathematical model of hydrolysis and transglycosylation catalyzed by cellobiase Gusakov, A. V.;A. P. Sintisyn;G. H. Goldstein;A. A. Kylosov
  30. Agric. Biol. Chem. v.49 Interaction of the β-transglycosylase of Trichoderma longibrachiatum with cellulose Tanaka, T;S. Oi