Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Streptomyces endus YP-1이 생산하는 항암활성 물질의 분리 및 정제

  • 최성원 (연세대학교 생명공학과, 생물산업소재연구센터) ;
  • 김병찬 (연세대학교 생명공학과, 생물산업소재연구센터) ;
  • 최선진 (연세대학교 생명공학과, 생물산업소재연구센터) ;
  • 김동섭 (밀양산업대 식품과학과) ;
  • 여익현 (풀무원 기술연구소) ;
  • 문순옥 (수원대학교 생명과학부) ;
  • 오두환 (연세대학교 생명공학과, 생물산업소재연구센터)
  • Published : 1997.10.01
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Abstract

Sulforhodamine B (SRB) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, RNA dot blot and Northern hybridization analysis were performed to screen microorganisms for the production of anticancer agent. Among microorganisms tested, strain YP-1 was selected for its cytotoxicity and ability to reduce the level of c-myc RNA. Strain YP-1 was identified as Streptomyces endus. The anticancer material produced by Streptomyces endus YP-1 was sequentially purified by solvent extraction, silica gel column chromatograpby, preparative TLC and preparative HPLC. The cancer material identified as azalomycin B by the instrumental analyses such as $^{1}$H-NMR, $^{13}$C-NMR, Mass, IR and UV absorption. It was colorless amorphous powder and its molecular weight was 1025.278. Azalomycin B, produced by Streptomyces endus YP-1, showed anticancer activity against several human cancer cell lines and reduction of c-Myc protein level in Colo320 DM cells which was determined by Western blot analysis.

Keywords

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