콩 발효식품에 있어서 페놀물질의 분리와 이의 항산화성

Antioxidative characteristics of isolated Crude Phenolics from Soybean Fermented Foods(Doenjang)

  • 이정수 (부산대학교 식품영양학과) ;
  • 최홍식 (부산대학교 식품영양학과)
  • 발행 : 1997.06.01

초록

Antioxidative characteristics of doenjang(fermented soybeans paste) phenolics on the lipid oxidation systems were studied by the determination of the oxidative related activity including lipoxygenase (LOX) inhibition, metal chelating and free radical scavengning of doenjang phenolics. Manlikong variety containing the highest amounts of phenolic compounds among the soybean variety, was used for doenjang processing. Doenjang was prepared by the series of processes including soaking for overnight, cooking for 1hr at 12Lb, first fermentation (3 days at 30$\pm$2$^{\circ}C$) for the preparation of meju(soybean koji) after inoculation of Asp. oryzae, and further fermentation(60 days at 30$\pm$2$^{\circ}C$) for the ripening after addition of salt 13% to meju. In order to investigate the antioxidative activity of phenolics in doenjang, the doenjang phenolics was extracted with methanol form freeze dried defatted doenjang. Antioxidative effects of methanol extract on linoleic acid oxidation system were observed by the significantly decreased levels of peroxide and conjugated diene formation. In addition, methanol extract resulted in the inhibition of LOX activity. and also, metal(FeCl$_3$) chelation and free radical scavengning activities were increased with increasing concentration of methanol extract.

키워드

참고문헌

  1. Am. J. Clin. Nutr. v.57 Lipid peroxidation : Its mechanism measurement, and significance Halliwell, B.;Chirico, S.
  2. J. Am. Oil Chem. Soc. v.52 Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene Branen, A. L.
  3. Phenolic compounds in food and their effects on health(Ⅱ), Am. Chem. Soc. Natural antioxidants from plant materials Pratt, D. E.;Huang, M. T.(eds.);Ho, S. T.(eds.);Lee. C. Y.(eds.)
  4. 서울대학교 박사학위 논문 지질과산화에 대한 maltol의 항산화효과에 관한 연구 윤여규
  5. 한국식품과학회지 v.22 칡뿌리의 항산화성분 오만진;이기순;손화영;김성열
  6. J. Biochem. v.26 Reductive inactivation of soybean lipoxygenase-1 by catechols, A possible machanism for regulation of lipoxygenase activity Kemel, C.;Flamberg, P. L.;Shorter, A. L.
  7. J. Food Sci. v.52 Effect of flavonoids and related compound on soybean lipoxygenase-1 activity King, D. L.;Klein, B. P.
  8. Lipids v.23 Relative inhibitory potencies of flavonoids on 12-lipoxygenase of fish gill Hsigh, R. J.;German, J. B.;Kinsella, J. E.
  9. J. Food Sci. v.43 Phenolic antioxidants of dried soybeans hammerschmidt, P. A.;Pratt, D. E.
  10. J. Agric. Food Chem. v.31 Stability of antioxidants formed from histidine and glucose by maillard reaction Lingnert, H.;Waller, G. R.
  11. 한국영양식량학회지 v.22 양조간장에서 분리한 갈색물질의 항산화성 최홍식;이정수;문갑순;박건영
  12. Official Method of Analysis.13th(ed.) A.O.A.C.
  13. 부산대학교 석사학위 논문 콩된장 담금 중 주요지방질 가수분해 효소의 활성과 지방질의 변화에 관한 연구 박혜숙
  14. J. Nurt. Sci. Vitaminol. v.30 Antioxidative components of sweet potatoes Hayase, F.;Kato, H.
  15. Official and Tentative Method of the American Oil Chemists Society. 2nd(ed.), Method Tila-64, Am. Oil. Chem. Soc. A.O.C.S.
  16. 부산대학교 석사학위논문 Penicillum sp. L-242H가 생산하는 lipoxygenase inhibitor에 관한 연구 황지숙
  17. Nature v.26 Antioxidant determination by the use of a stable free radical Blois, M. S.
  18. J. Agric. Food Chem. v.22 Soybean isoflavones, characterization, determination and antifungal activity Naim, M.;Gestetner, B.;Zilkah, S.;Birk, Y.;Bondi, A.
  19. 한국식품과학회지 v.26 국내산 식물성 식품중 폐놀성 물질의 함량분석 이정희;이서래
  20. Cereal Chem. v.49 Evaluation of methods for tannin analysis in sorghum grains Maxson, E. D.;Rooney, L. W.
  21. J. Agric. Food Chem. v.33 Polyphenols in mung bean(Virginia radidata(L.) Wilczek);Determination and removal Barroga, C. F.;Laurena, A. C.;Mondoza, E. M. T.
  22. Food antioxidants The mechanism of antioxidant action in vitro Goldon, M. H.;Hudson, B. J. F.(ed.)
  23. Phytochemistry v.24 Inhibition of lipoxygenase-dependent lipid peroxidation by quercetin, machanism of antioxidative function Takahama, U.
  24. 한국영양식량학회지 v.22 양조간장에서 분리한 melanoidin 관련물질의 항산화작용 특성 최홍식;이정수;이창용
  25. 한국식품과학회지 v.21 항산화제 또는 팜유로 보강된 미강유를 이용한 라면의 산화안정성 강동호;박혜경;김동훈
  26. Lipids v.27 Redox cycling iron and lipid peroxidation Minotti, G.;Aust, S. D.
  27. J. Nutr. Sci. Vitaminol. v.38 Japanese soybean paste miso scavenges free radicals and inhibits lipid peroxidation Santiago, L. A.;Hiramatsu, M.;Mori, A.