Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지

Genotoxicological safety of the Gamma-Irradiated Medicinal Herbs in the Micronucleus Test Using CHO Cells In Vitro

CHO 세포에서의 소핵시험을 이용한 감마선조사 생약재의 안전성에 관한 유전독성학적 평가

  • 조성기 (한국원자력연구소 방사선식품공학연구팀)
  • Published : 1997.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

The three medicinal herbs-Curcuman longa Linne, Paeonia japonica Miyabe, Scutellaria baikalensis George-irradiated with gamma rays were tested for their possible genotoxicity. The methanol-soluble and water-soluble fractions of the 10kGy gamma-irradiated herbs were examined in cultured Chinese hamster ovary(CHO) cells for their ability to induce micronuclei. No mutagenicity of each test material was detected in the assay with or without metabolic activation. The safety of the herbs irradiated with gamma rays at practical doses needs further investigations using in vivo genotoxicity and chronic and reproductive toxicity tests.

Keywords

References

  1. Food irradiation, Up-to-date status Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture Ahmed, M.
  2. 동위원소회보 v.9 식품산업에서 원자력 기술의 이용 변명우
  3. J. Food Sci. Nutr. v.1 Status and prospects of food irradiation technology in Korea Byun, M. W.;Yook, H. S.;Jo, S. K.;Chong, Y. J.
  4. Wholesomeness of irradiated food Report of a Joint FAO/IAEA/WHO expert committee on the wholesomeness of irradiated food. Technical Report Series WHO
  5. Food irradiation The position of the World Health Organization. 36th General Conference of the International Atomic Energy Agency, Scientific session Daferstein, F. K.
  6. Mutation Res. v.147 Measurement of micronuclei in lymphocytes Fenecdh, M.;Morley, A. A.
  7. Appl. Radiat. Jsot. v.34 The present state and perspectives of micronucleus assay in radiation protection, A review Almassy, Z.;Krepinsky, A. B;Bianco, A.;Koteles, G. J.
  8. Mutation Res. v.130 The in vitro micronucleus assay for detection of cytogenetic effects induced by mutagen-carcinogens comparison with the in vitro sister-chromatid exchange assay Lasne, C.;Gu, Z. W.;Venegas, W.;Chouroulinkov, I.
  9. Mutation Res v.319 Cytogenetic toxicity of uranyl nitrate in Chinese hamster ovary cells Lin, R. H.;Wu, L. J.;Lee, C H;Lin-Shiau, S. Y.
  10. Mutation Res v.190 Measurement of micronuclei by cytokinesis-block method in cultured Chinese hamster cells : comparison with types and rates of chromosome aberrations Wakata, A.;Sasaki, M S
  11. Environ. Mol. Mutagen v.10 A modified mouse peripheral blood lymphocyte culture system for cytogenetic analysis Erexson, G. L.;Kligerman, A. D.
  12. Mutation Res. v.312 Micronuclei and 3AB index in human and canine lymphocytes after in vitro X-irradiation Catena, C.;Conti, D.;Villani, P.;Nastasi, R.;Archilei, R.;Righi, E.
  13. 한국식품위생 · 안전성학회지 v.9 방사선조사 인삼의 유전독성에 관한 연구 하광원;정해관;오혜영;허옥순;손수정;한의식;정성칠;최부영;김영미;김필선;문화회
  14. Principles and methods of toxicology(3rd ed.) Genetic toxiology Brusick, D;Hayes, A. W(ed.)
  15. Radiat. Res. v.115 A cytogenetic comparison of the responses of mouse and human perpheral blood lymphocytes to $^{60}Co$ γ radiation Kligerman, A. D;Halperin, E. C.;Erexson, G. L.;Honore, G.;Westvrook-collins, B;Allen, J. W.
  16. Mutation Res. v.225 Acentric chromosomal fragments, and micronuclei the displacement factor Savage, J. R. K.