Effect of White Ginseng on the Function of Mouse Peritoneal Macrophages and their Gene Expression

백삼성분이 마우스 복강 탐식세포의 기능 및 유전자 발현에 미치는 영향

  • 배지현 (계명대학교 식품영양학과)
  • Published : 1997.12.01

Abstract

In order to investigate the immunomodulatory mechanism of white ginseng, the effects of total saponin of Ginsenoside Rb$_2$component on the phagocytosis and reactive oxygen intermediate(ROI) production of mouse peritoneal macrophages were studied. Both phagocytosis assay nitrobluetetrazolium reduction test showed 20$\mu\textrm{g}$/ml concentration of total saponin significantly increased the activity of phagocytosis and production of ROI. Also cytokine gene expression of the macrophages was analyzed using reverse transcription polymerase chain reaction. In the RT-PCR assay, 20$\mu\textrm{g}$/ml concentration of either total saponin or Ginsenoside Rb$_2$increased IL-1 and TNF expression of the macrophages.

Keywords

References

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