Effect of Chitosan and N. O-Carboxymethyl Chitosan of Different Sources and Molecular Weights on Lipid Metabolism

급원과 분자량이 다른 Chitosan 과 N, O-Carboxymethyl Chitosan이 지방대사에 미치는 영향

  • 배계원 (이화여자대학교 식품영양학과)
  • Published : 1997.09.01

Abstract

This study was performed to investigate the effect of chitosan and NOCC from different sources and of different molecular weights on lipid metabolism. Sprague-Dawley rats were blocked into 26 groups according to body weight, and were raised for 4 weeks. Cadmium chloride was given at the level of 0 or 400 ppm in diet. Various chitosan and NOCC sources were given at the level of 0%(w/w) of diet. Total serum cholesterol and serum triglyceride concentrations were little affected by chitosan and NOCC supplements. However, HDL-cholesterol concentration and HDL : total cholesterol ratio were increased , and liver lipid, cholesterol , and triglyceride concentration were decreased by chitosan and NOCC supplements. The cholesterol and lipid lowering activity depends on fiber sources(crab and shrimp); type (chitosan and NOCC); and molecular weight(low , medium , and high). Among cadmium-free groups, chitosan-fed groups showed greater activity than NOCC -fed groups in lowering cholesterol and lipid levels, and greater fecal excretion of lipids and bile acids. Crab chitosans were more effective in cholesterol and lipid lowering activity than shrimp chitosans. The group fed high molecular weight crab chitosan showed the highest fecal excretion of lipids and bile acids. This indicated that high molecular weight crab chitosan was most effective in interfering with cholesterol and lipid absorption.

Keywords

References

  1. CRC Handbook of Dietary Fiber in Human Nutrition Dietary fiber-A Perspctive Ruth Mcperson;G.A.Spiller(ed.)
  2. J Am Med Assoc v.229 Dietary fiber and tissue Burkitt,D.P.;Walker,A.R.P.;Painter,N.S.
  3. Am J Clin Nutr v.25 Ischemic heart disease and dietary fiber Trowell,H.
  4. Rev Eur Etud Clin Biol Res v.17 Dietary fiber and coronary heart disease Trowell,H.
  5. Proc Nutr Soc v.32 Dietary fiber, ischemic heart disease and diabetes mellitus Trowell,H.
  6. Lancet v.1 Dietary fiber redefined Trowell,H.
  7. Lancet v.1 Dietary fiber redefined Godding,E.W.
  8. Unconventional Sources of Dietary Fiber Aminopolysaccharides-Their potential as dietary fiber Furda,I.;Furda,I.(ed.)
  9. Chitin Muzzarrelli,R.A.A.
  10. U.S. Patent #4.619.955 Patents pending in Australi, Canada, France, Germany, Italy, Japan, the Netherlands and the United Kingdom N,O-carboxymethyl chitosan and preparative method therefor Hayes,E.R.
  11. Nutr Rev v.49 Soluble fiber polysaccharides : Effects on plasma cholesterol and colonic fermentation Topping,D.L.
  12. Nutr Rept Int v.18 Hypocholesterolemic effects of chitosan in cholesterol-fed rats Sugano,M.;Fujikawa,T.;Hiratsuji,Y.;Hasegawa,Y.
  13. Am J Clin Nutr v.33 A novel use of chitosan as a hypocholesterolemic agent in rats Sugano,M.;Fujikawa,T.;Hiratsuji,Y.;Nakashima,K.;Fukuda,N.;Hasagawa,Y.
  14. Nutr Rept Int v.20 Hypolipidemic activity of chitosan and other polysaccharides in rats Nagyvary,J.J.;Falk,J.D.;Hill,M.L.;Schmidt,M.L.;Wilkins,A.K.;Bradbury,E.L.
  15. Advances in Chitin and Chitosan N,O-carboxymethyl chitosan, a new water soluble chitin dervative Davis,D.H.;Elson,C.M.;Hayes,E.R.;Brine,C.J.(ed.);Sandford,P.A.(ed.);Zikakis,J.P.(ed.)
  16. Chitin, chitosan 및 chitosan 유도체가 흰쥐의 cadmium중 독과 지방대사에 미치는 영향 배계현;김호정;김미경
  17. Fed Proc v.41 Dietary fiber, lipid metabolism, and atherosclerosis Vahouny,G.V.
  18. Am J Clin Nutr v.29 The binding of the components of mixed micell to dietary fiber Eastwood,M.A.;Mowbray,L.
  19. Food Technol v.23 Use of chitinous polymers in food-A challenge for food research and development Knorr,D.
  20. Lipids v.23 Hypocholesterolemic action of chitosans with different viscosity in rats Sugano,M.;Watanabe,S.;Kishi,A.;Izume,M.;Ohtakara,A.
  21. Endocrinology v.132 Some effects of chitosan on liver function in the rat Lehoux,J.G.;Grondin,F.
  22. Proceeding of Annual Meeting of Japanese Society of Nutrition and Food Science Abstract Ishii,T.;Takeuchi,S.;Tsugita,T.
  23. Can J Biochem Physiol v.37 A rapid method of total lipid extraction and purification Bligh,E.G.;Dyer,W.J.
  24. Am J Clin Pathol v.24 Rapid estimation of free and total cholesterol Zak,B.
  25. Clin Chem v.21 A manual colorimetric assay of triglyceride in serum Biggs,H.G.;Erickson,J.M.;Moorehead,W.R.
  26. Proc Soc Exp Biol Med v.143 Effect of cholestyramine on fecal bile salt excretion in rats fed diets containing mudium chain triglycerides or corn oil Hagermann,L.M.;Schneider,D.L.
  27. Scand J Gastroent v.9 Quantitative determination of bile acids and their conjugates using thin-layer chromatography and a purified 3α-hydroxy steroid dehydrogenase Fausa,O.;Skalhegg,B.A.
  28. Principels and procedures of stastics Steel,R.G.D.;Torrie,J.H.
  29. Proc Soc Exp Biol Med v.189 A comparison of the lipid lowering and intestinal morphological effects of cholestylamine, chitosan and oat gum in rats(42773) Jennings,C.D.;Boleyn,K.;Bridges,S.R.;Wood,P.J.;Anderson,J.W.
  30. Am J Clin Nutr v.38 Comparative dffects of chitosan and cholestyramine on lymphatic absorption of lipid in the rat Vahouny,G.V.;Satchithanandam,S.;Cassidy,M.M.;Lightfoot,F.B.;Furda,I.
  31. Handbook of Dietary Fiber in Human Nutrition Influence of dietary fiber on cholesterol metabolism in experimental animals Kritchevsky,D.;Strory,J.A.
  32. 한국영양학회지 v.23 종류가 다른 고 섬유질식이가 흰쥐의 납 흡수 및 체내 대사에 미치는 영향 김미경;이혜영
  33. 한국영양학회지 v.27 Chitin과 Chitosan이 흰쥐의 Cadmium중독과 지방대사에 미치는 영향 김미경;설은영
  34. Am J Cardiol v.62 Effects of solid and liquid guar gum on plasma cholesterol and triglyceride concentration in moderate hypercholesterolemia Superko,H.R.;Haskell,W.L.;Sawrey-Kubicek,L.;Faquhar,J.W.
  35. Br J Nutr v.68 Relationship between structure and function of dietary fibre : A comparative study on the effects of three galactomannans on cholesterol metabolism in the rat Evans,A.J.;Hood,R.L.;Oakenfull,D.G.;Sidhu,G.S.
  36. J Agric Food Chem v.41 Effects of chitosan hydrolysates on lipid absorption and on serum and liver lipid concentration in rats Ikeda,I.;Sugano,M.;Yoshida,K.;Sasaki,E.;Iwamoto,Y.;Hatano,K.