Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Chitosan Production from Mutant of Cunninghamella blakesleeana IFO 4443

Cunninghamella blakesleeana IFO 4443의 변이주로부터 키토산의 생산

  • 류병호 (경성대학교 식품공학과) ;
  • 김희숙 (경성대학교 식품공학과) ;
  • 원용돈 (부경대학교 고분자공학과) ;
  • 임복규 (부산지방식품의약품청)
  • Published : 1997.02.01
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Abstract

A method for lab scale production and isolation of chitosan from mycelia of Cunninghamella blakesleeana IFO 4443 mutant was developed. Mutant of Cunninghamella blakesleeana IFO 4443-10 obtained by W radiation was cultivated in 5L jar fermentor at 3$0^{\circ}C$ for 3days. The fungus were grown well at pH 4.5 Chitosan was readily extracted from mycelia walls with alkali treatment. The maximum yield of chitosan obtained was 1012mg/L and degree of deacetylation was 84.6%.

키토산을 생산하기 위하여 Cunninggamella blak-esleeana IFO 4443을 자외선조사하여 생성능이 우수한 Cunningmella blakesleeana IFO 4443-10의 변이주를 분리하였다. 본 변이주인 C. blakesleeana IFO 4443-10을 3$0^{\circ}C$에서 pH 4.5에서 3일간 발효하여 mycelia 발효액을 알칼리로 처리하여 chitosan을 얻었다. 이때 chitosan의 수율은 1012mg/l로 높았으며 탈아세틸화는 84.6% 이였다.

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References

  1. シ-ェムシ キチソ,キトサシの利用. 天然高分子の 最新技術 平野茂博
  2. フトケミカル v.11 食品素材として キチソ, キトサソ 硏究の現狀と 將來性 平野茂博
  3. Petrotech v.15 夫利用 べイォマス資源, キチソ高度有效利用ヘの開發 栗田惠轉
  4. Proc. Ist Intern. Conf. chitin/chitosan Allan, G. C.;Fox, J. R.;Knog, N.;Muzzareli, R. A. A.;Pariger, E. R. (eds.)
  5. フトケミカル v.11 キチソ, キトサソの生理活性につぃて 戶倉淸一
  6. Bioprocess Technol. v.9 Chitin and its derivative : Opportunities in diverse Niches, $335 million U.S. market foreseen Anon
  7. Chitin and chitosan Potential of acid-soluble and water-soluble chitosans in biotechnology Knorr, D.;Beaumint, M. D.;Pandya, Y.;Anthonsen, T.;Sanford, P.(eds.)
  8. Ann. Rev. Microbiol. v.22 Cell wall chemistry morphogenesis and taxonomy of fungi Bartnicki-Garcia, S.
  9. Appl. Environ. Microbiol. v.38 Production and isolation of chitosan from Mucor rouxii White, S. A.;Farina, P. F.;Pulton, I.
  10. Process Biochem. v.19 Chitosan by fermentation McGahren, W. J.;Perkinson, G. A.;Growich, J. A.;Leese, R. A.;Ellestad, G. A.
  11. Biotechnol. Lett. v.8 Production and conversion of chitosan with cultures of Mucor rouxii and Phycomyces blakesleeanus Knorr, D.;Klein, J. K.
  12. キチソ,キトサソ 硏究會編 キチソ, キトサソ 實驗 マニュアル. 戶創淸一
  13. Biotechnol. Lett. v.12 Chitosan production from Rhizopus oryzae mycelia Hwang, Y. D.
  14. Nippon Nogeikagaku Kaishi v.66 Effect of the cultivation pH on the degree of deacetylatino of chitosan produced by Rhizopus acetoinus HUT 1219 Kubo, T.;Yoshihara, K.;Hasokawa, J.;Nishiyama, M.