Journal of Microbiology and Biotechnology
- Volume 7 Issue 3
- /
- Pages.197-199
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- 1997
- /
- 1017-7825(pISSN)
- /
- 1738-8872(eISSN)
Production of Indigo and Indirubin by Escherichia coli Containing a Phenol Hydroxylase Gene of Bacillus stearothermophilus
- Kim, In-Cheol (Department of Microbiology, Michigan State University) ;
-
Chang, Hae-Choon
(Department of Food and Nutrition, Chosun University)
;
- Oriel, Patrick (Department of Microbiology, Michigan State University)
- Published : 1997.06.01
Abstract
Escherichia coli recombinants containing the cloned phenol hydroxylase gene of Bacillus stearothermophilus BR219 were shown to produce both indigo and its structural isomer indirubin during culture on LB broth. The ratio of indirubin/indigo was highest under conditions of prolonged culture and reduced culture oxygenation.