Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Enhanced Production of Maltotetraose-producing Amylase by Recombinant Bacillus subtilis LKS88 in Fed-batch Cultivation

  • KIM, DAE-OK (Department of Food Science & Technology and Research Center for New Bio-Materials in Agriculture, Seoul National University) ;
  • KYUNGMOON PARK (Department of Food Science & Technology and Research Center for New Bio-Materials in Agriculture, Seoul National University) ;
  • JAE-WOOK SONG (Department of Food Science & Technology and Research Center for New Bio-Materials in Agriculture, Seoul National University) ;
  • JIN-HO SEO (Department of Food Science & Technology and Research Center for New Bio-Materials in Agriculture, Seoul National University)
  • Published : 1997.12.01
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Abstract

Recombinant Bacillus subtilis LKS88[pASA240] containing the amylase gene from Streptomyces albus KSM-35 was exploited in fed-batch cultivation for mass production of maltotetraose-producing amylase. The effects of dissolved oxygen, additional organic nutrients (peptone and yeast extract) and mixed carbon sources (glucose plus soluble starch) on amylase production were examined in fed-batch operations in an effort to determine the optimum conditions for a maximum amylase productivity. Under the optimum conditions, maximum amylase activity was about 4.2 times higher than that obtained in batch cultivations, indicating that mass production of maltotetraose-producing amylase could be accomplished in fed-batch cultivation of the recombinant B. subtilis strain.

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