Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Analysis of Kasugamycin in Pesticide Formulations by Reversed-Phase Ion Pair Liquid Chromatography

역상 이온쌍 액체크로마토그래피에 의한 농약 제품 중 Kasugamycin의 분석

  • Kim, Taek-Jae (Department of Chemistry, Kyonggi University) ;
  • Kim, Kyong-Sun (Division of Pesticide Quality, Department of Crops Protection, National Institute Agricultural Science & Technology) ;
  • Yoon, Chae-Hyuk (Division of Pesticide Quality, Department of Crops Protection, National Institute Agricultural Science & Technology) ;
  • Joo, Jin-Bok (Division of Pesticide Quality, Department of Crops Protection, National Institute Agricultural Science & Technology) ;
  • Kim, Chung-Hyo (Division of Pesticide Quality, Department of Crops Protection, National Institute Agricultural Science & Technology)
  • 김택제 (경기대학교 이과대학 화학과) ;
  • 김경선 (농업과학기술원 작물보호부 농약품질과) ;
  • 윤채혁 (농업과학기술원 작물보호부 농약품질과) ;
  • 주진복 (농업과학기술원 작물보호부 농약품질과) ;
  • 김충효 (농업과학기술원 작물보호부 농약품질과)
  • Received : 1997.04.10
  • Published : 1997.10.25
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Abstract

Analytical method using the reversed phase ion-pair chromatography (RP-IPC) for the determination of kasugamycin(5-amino-2-methyl-6-(2,3,4,5,6-pentahydroxy cyclohexyloxy)tetrahydropyran-3-yl-amino-${\alpha}$-imino acetic acid), pesticide as fungicide bactercide has been established. The retention behavior of kasugamycin in the RP-IPC was examined with respect to the effect of concentrations of organic modifiers, pH of eluent and types and concentrations of the counter ions as ion-pair reagent. This method developed by the optimum factors, can be used for the application of the quality control in the crude product and its formulation.

역상 이온쌍 고성능액체크로마토그래피(RP-IPC)에 의한 살균제 농약 kasugamycin(5-amino-2-methy-6-(2,3,4,5,6-pentahydroxy cyclohexyloxy) tetrahydropyran-3-yl-amino-${\alpha}$-iminoacetic acid)의 정량분석법을 확립하였다. Ksugamycin의 정량분석법을 확립하기 위해 RP-IPC에서 organic modifier의 농도, 용리액의 pH 및 상대이온(counter ion)의 종류와 농도에 의한 시료의 머무름 거동에 대한 영향을 조사 검토하였다. 확립된 최적 변수를 바탕으로 개발된 이 분석법은 kasugamycin의 품질관리에 활용할 수 있음을 확인하였다.

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