Congenital transmission of Theileria sergenti in cattle verified by immunohistochemistry

소 Theileria sergenti의 태반감염에 대한 면역세포화학적 증명

  • Baek, Byeong-kirl (College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Jin-ho (College of Veterinary Medicine, Chonbuk National University) ;
  • Onuma, Misao (Faculty of Veterinary Medicine, Hokkaido University)
  • Received : 1997.08.05
  • Published : 1997.12.25

Abstract

The spleen and placenta from the aborted fetuses as well as lymphnodes and placenta of the corresponding dams naturally infected with T sergenti were used to localize the parasite antigens by immunohistochemical staining for the possible congenital transmission of theileriosis. Parasite-specific antigens were detected immunohistochemically by incubating the sections with specific monoclonal antibody prepared against 34KD surface antigen of T sergenti and visualized via the avidin biotin complex(ABC) method. Specific T sergenti antigen was detected in the sections of formalin or acetone-fixed fetal spleens and placenta. Similar antigens were also demonstrated in lymphnodes and placentas of the corresponding dams. It is concluded that this technique will eventually play an important role in specialized diagnostic laboratories in the verification/evaluation of congenital infection with T sergenti.