BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Characterization of the Stearic Acid-Induced Uncoupling of Mitochondrial Respiration

  • Chun, Sun-Bum (Department of Biochemistry, Kangwon National University) ;
  • Ho, Sung-Sook (Department of Biochemistry, Kangwon National University) ;
  • Han, Sang-Hwa (Department of Biochemistry, Kangwon National University)
  • Received : 1997.01.14
  • Published : 1997.03.31
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Abstract

In order to assess controversial' proposals concerning the fatty acid-induced uncoupling of mitochondrial oxidative phosphorylation, we investigated the interaction of stearic acid with key mitochondrial proteins and measured the effect of stearic acid on the respiration of cytochrome c oxidase vesicles. Electron paramagnetic resonance spectra of spin-labeled stearic acid clearly demonstrated that cytochrome c oxidase interacts strongly with stearic acid. However, the respiration of detergent-solubilized cytochrome c oxidase was not altered significantly by stearic acid. Surprisingly, adenine nucleotide carrier, which was assumed to bind and translocate fatty acid anions in the Skulachev model of uncoupling, did not bind stearic acid at all. The respiration rate of cytochrome c oxidase vesicles was increased by ~70% in the presence of $20{\mu}m$ stearic acid and this uncoupling was attributed to a simple protonophoric effect of stearic acid.

Keywords

References

  1. Eur. J. Biochem. v.182 Andreyev, A.Yu.;Bondareva, T.O.;Dedukhova, V.I.;Mokhova, E.N.;Skulachev, V.P.;Tsofina, L.M.;Volkov, N.I.;Vygodina, T.V. https://doi.org/10.1111/j.1432-1033.1989.tb14867.x
  2. J. Biol. Chem. v.269 Brustovetsky, N.;Klingenberg, M.
  3. FEBS Lett. v.295 Dedukhova, V.I.;Mokhova, E.N.;Skulachev, V.P.;Starkov, A.A.;Arrigoni-Martelli, E.;Bobyleva, V.A. https://doi.org/10.1016/0014-5793(91)81382-I
  4. Biochemistry v.27 Drees, M.;Beyer, K. https://doi.org/10.1021/bi00423a012
  5. J. Biol. Chem. v.271 Garlid, K.D.;Orosz, D.E.;Modriansky, M.;Vassanelli, S.;Jezek, P. https://doi.org/10.1074/jbc.271.5.2615
  6. Biochemistry v.28 Gregory, L.;Ferguson-Miller, S. https://doi.org/10.1021/bi00432a044
  7. Biochemistry v.29 Horvath, L.I.;Drees, M.;Beyer, K.;Klingenberg, M.;Marsh, D. https://doi.org/10.1021/bi00499a013
  8. FEBS Lett. v.343 Jezek, P.;Freisleben, H.J. https://doi.org/10.1016/0014-5793(94)80599-7
  9. FEBS Lett. v.361 Jezek, P.;Bauer, M.;Trommer, W.E. https://doi.org/10.1016/0014-5793(95)00201-J
  10. Biochemistry v.32 Kamp, F.;Hamilton, J.A. https://doi.org/10.1021/bi00092a017
  11. FEBS Lett. v.336 Knke, D.;Ludwig, B.;Kadenbach, B. https://doi.org/10.1016/0014-5793(93)81616-8
  12. Methods Enzymol. v.125 Krer, R.
  13. Biochem. J. v.254 Labonia, N.;Mler, M.;Azzi, A. https://doi.org/10.1042/bj2540139
  14. Biochemistry v.26 Luvisetto, S.;Pietrobon, D.;Azzone, G.F. https://doi.org/10.1021/bi00397a021
  15. Biochemistry v.22 Munding, A.;Beyer, K.;Klingenberg, M. https://doi.org/10.1021/bi00277a031
  16. Biochem. Int. v.17 Nagami, M.;Yoshida, S.;Saitoh, T.;Takeshita, M.;Ogawa, T.
  17. J. Biochem. Mol. Biol. (formerly Korean Biochem. J.) v.29 Park, N.H.;Chun, C.B.;Han, T.Y.;Han, S.
  18. Biochemistry v.25 Rottenberg, H.;Hashimoto, K. https://doi.org/10.1021/bi00355a045
  19. EMBO J. v.6 Runswick, M.J.;Powell, S.J.;Nyren, P.;Walker, J.E.
  20. FEBS Lett. v.382 Ruzicka, M.;Borecky, J.;Hanus, J.;Jezek, P. https://doi.org/10.1016/0014-5793(96)00161-5
  21. FEBS Lett. v.264 Schfeld, P. https://doi.org/10.1016/0014-5793(90)80259-L
  22. FEBS Lett. v.303 Schfeld, P. https://doi.org/10.1016/0014-5793(92)80516-J
  23. Biochim. Biophys. Acta v.1228 Shinohara, Y.;Unami, A.;Teshima, M.;Nishida, H.;van Dam, K.;Terada, H. https://doi.org/10.1016/0005-2728(94)00179-9
  24. FEBS Lett. v.294 Skulachev, V.P. https://doi.org/10.1016/0014-5793(91)80658-P
  25. FEBS Lett. v.251 Thiel, C.;Kadenbach, B. https://doi.org/10.1016/0014-5793(89)81469-3
  26. FEBS Lett. v.349 Vianello, A.;Petrussa, E.;Macri, F. https://doi.org/10.1016/0014-5793(94)00712-8
  27. Biochim. Biophys. Acta v.1183 Wojtczak, L.;Schfeld, P. https://doi.org/10.1016/0005-2728(93)90004-Y
  28. FEBS Lett. v.364 Woldegiorgis, G.;Lawrence, J.;Ruoho, A.;Duff, T.;Shrago, E. https://doi.org/10.1016/0014-5793(95)00375-J
  29. J. Biol. Chem. v.236 Yonetani, T.