BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Characteristics of the Inhibitory Action of Protease Inhibitors on the Glucose-6-phosphate Transporter

  • Choi, Joon-Sig (Center for Molecular Catalysis, Department of Chemistry, Seoul National University) ;
  • Shin, Jeong-Sook (Center for Molecular Catalysis, Department of Chemistry, Seoul National University) ;
  • Choi, Hong-Sug (Center for Molecular Catalysis, Department of Chemistry, Seoul National University) ;
  • Park, Jong-Sang (Center for Molecular Catalysis, Department of Chemistry, Seoul National University)
  • Received : 1997.02.03
  • Published : 1997.03.31
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Abstract

The present paper reports characteristics and specificity of the inhibitory action of $N^{\alpha}-tosyl-L-lysine-chloromethyl\;ketone$ (TLCK) and $N^{\alpha}-tosyl-L-phenylalanine-chloromethyl\;ketone$ (TPCK) on the glucose6-phosphate transporter of rat liver microsomes. The TLCK-induced inhibition was pH dependent. The inhibition constants for TPCK were determined by following pseudo-Lst order reaction mechanism. The inhibition was protected by preincubation with excess amount of glucose-6-phosphate. The results proved that (a) TLCK inactivates the microsomal glucose-6-phosphate transporter, (b) the inhibition results from the modification of sulfhydryl groups of the transporter.

Keywords

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