BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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The Action of Hepatitis B Virus Enhancer 2-Core Gene Promoter in Non-Viral and Retroviral Vectors for Hepatocyte-Specific Expression

  • Rih, Jeong-Keun (Department of Microbiology, Seoul National University) ;
  • Oh, Sang-Taek (Department of Microbiology, Seoul National University) ;
  • Hwang, Deog-Su (Institute for Molecular Biology and Genetics, Seoul National University) ;
  • Kim, Sun-Young (Institute for Molecular Biology and Genetics, Seoul National University) ;
  • Yim, Jeong-Bin (Department of Microbiology, Seoul National University)
  • Received : 1997.05.15
  • Published : 1997.07.31
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Abstract

Heptocvte-specific expression induced by Hepatitis B virus (HBV) enhancer 2-core gene promoter was examined in various hepatocyte and non-hepatocyte cell lines. using non-viral and retroviral vector systems in which chloramphenicol acetyltransferase (CAT) is used as a reporter. The non-viral plasmid containing the HBV enhancer 2-core promoter exhibited 22 and 66% of CAT activities in hepatoma cell lines. HepG2 and Hep3B, respectively when compared with CAT activity expressed by CMV promoter. The CAT activities, however. were found to be marginal in other tested hepatoma cell lines as well as mouse primary hepatocytes and non-hepatocytes. The HBV enhancer 2 located upstream the CMV promoter did not affect the CMV promoter activity nor provided hepatocyte-specific expression. Transfection of retroviral plasmid DNA containing the HBV enhancer 2-core promoter as an internal promoter exhibited high and specific CAT expression in HepG2 and Hep3B cell lines but the activity value was 5 to 10 fold lower than the non-viral plasmid with identical promoter. These results suggest that the usage of HBV enhancer 2-core promoter for liver specific expression is limited to certain vectors and hepatocyte cell lines.

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