BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Preliminary X-Ray Diffraction Study of Glutathione S-Transferase from Pseudomonas sp. DJ77

  • Choi, Heung-Soo (Department of Biochemistry, Chungbuk National University) ;
  • Woo, Ju-Rang (Department of Biochemistry, Chungbuk National University) ;
  • Lee, Jung-Hee (Department of Biochemistry, Chungbuk National University) ;
  • Chung, An-Sik (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Ryu, Seong-Eon (Protein Engineering Research Group, Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Kim, Young-Chang (Department of Microbiology, Chungbuk National University) ;
  • Chung, Yong-Je (Department of Biochemistry, Chungbuk National University)
  • Received : 1997.04.29
  • Published : 1997.07.31
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Abstract

A bacterial glutathione S-transferase from Pseudomonas sp. DJ77 has been crystallized. The crystals diffract to at least $2.3\;\AA$ resolution, and belong to the orthorhombic space group $P2_{1}2_{1}2_{1}$, with cell parameters $a=97.4\;\AA,\;b=100.3\;\AA$, and $c=46.0\;\AA$. There is one dimer molecule of pGST per crystallographic asymmetric unit. with the crystal volume per protein mass of $2.34\;\AA^3/dalton$ and a solvent content of about 47% (v/v).

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