Cytotoxic Effect of the Pine needle extracts

솔잎 추출물의 in vitro계 암세포 성장억제효과

  • Kim, Eun-Jeong (Division of Food and Biotechnology, Kangwon National University) ;
  • Jung, Sung-Won (Division of Food and Biotechnology, Kangwon National University) ;
  • Choi, Keun-Pyo (Division of Food and Biotechnology, Kangwon National University) ;
  • Ham, Seung-Shi (Division of Food and Biotechnology, Kangwon National University) ;
  • Gang, Ha-Yeong (Forestry Research Institute)
  • 김은정 (강원대학교 식품생명공학부) ;
  • 정성원 (강원대학교 식품생명공학부) ;
  • 최근표 (강원대학교 식품생명공학부) ;
  • 함승시 (강원대학교 식품생명공학부) ;
  • 강하영 (임업연구원)
  • Published : 1998.02.01

Abstract

This study was performed to observe cytotoxic effect of the pine needle extracts against cancer cell lines including human gastric carcinoma (KATOIII), human lung carcinoma (A549), human hepatocellular carcinoma (Hep3B) and human breast adenocarcinoma (MCF-7) using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) and SRB (sulforhodamine B) method. The extracts were prepared by step-wise fractionation of ethanol extract of pine needles using diethylether, chloroform, ethylacetate, butanol and water. The growth of the cancer cells in medium containing pine needle extracts were significantly inhibited degree in proportion to the increase of the extract concentration. A significant shrinkage of Hep3B cells was observed when the cells were exposed into 0.5, 1 mg/mL of pinus rigida extract.

곰솔, 리기다, 잣나무, 적송잎의 에탄올 추출물들은 농도가 증가함에 따라 폐암, 간암, 위암, 유방암 세포에 대한 성장억제율이 증가함을 보여 주였고 각각의 디에틸에테르, 클로로포름, 에틸 아세테이트, 부탄올 및 수층 분획물 또한 농도 증가에 따라 성장 억제율이 증가하였다. 그러나 0.25 mg/mL 처리시 각각의 비교 결과 잣나무의 디에틸에테르 분획물은 위암세포에 대해서는 억제효과가 보여지지 않았다. 현미경의 관찰하에서 암세포의 변화는 세포막의 경계가 흐트러지는 사멸 현상을 보였다.

Keywords