한국식품과학회지 (Korean Journal of Food Science and Technology)
- 제30권6호
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- Pages.1464-1469
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- 1998
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- 0367-6293(pISSN)
SOS Chromotest 및 Ames test에서의 Chloropropanol류의 변이원성
Mutagenicity of Chloropropanols in SOS Chromotest and Ames Test
- Song, Geun-Seoup (Department of Food Engineering, Iri National College of Agriculture and Technology) ;
- Han, Sang-Bae (Seoul FDA) ;
- Uhm, Tae-Boong (Faculty of Biological science, Chonbuk National University) ;
- Choi, Dong-Seong (Division of Biotechnology and Environmental engineering, Woosuk University)
- 발행 : 1998.12.01
초록
3-Chloro-1,2-propanediol (3-MCPD), 1,3-dichloro-2-propanol (1,3-DCP), 2,3-dichloro-1-propanol (2,3-DCP)과 같은 chloropropanol들이 일부 식품에서 검출됨으로서 위해성 논란이 제기되었기에 본 연구에서는 Ames test와 SOS Chromotest를 이용하여 미생물 시험계에서의 chloropropanol류의 유전독성 및 변이원성을 검토하였다. E. coli PQ37에서는 1,3-DCP를 제외한 3-MCPD와 2,3-DCP는 뚜렷한 용량반응 관계를 보이며 SOS반응 유도활성을 나타내어 유전독성이 있는 것으로 판단되었고 3-MCPD보다는 2,3-DCP가 강한 유도활성을 나타내었다. 또한 E. coli PQ35 (PQ37
SOS Chromotest and Ames test were carried out to evaluate the mutagenicity of three chloropropanols. In the SOS Chromotest, 3-monochloro-l,2-propanediol (3-MCPD) and 2,3-dichloro-1-propanol (2,3-DCP) except for 1,3-dichloro-2-propanol (1,3-DCP) induced SOS response in Escherichia coli PQ37 with dose-response relationship and 2,3-DCP was far more genotoxic than 3-MCPD. The genotoxic activities of both compounds, however, were very lower in E. coli PQ35 (PQ37