The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

Protective effect of Salviae-radix extraction in $H_2O_2$ induced renal cell injury

$H_2O_2$에 의한 신장(腎臟) 세포 손상에 대한 단삼(丹參) 추출물의 방지 효과

  • Kim, Sang-Beum (Dept. of Internal Medicine, College of Oriental Medicine, Dong-guk University) ;
  • Jeong, Ji-Cheon (Dept. of Internal Medicine, College of Oriental Medicine, Dong-guk University)
  • 김상범 (동국대학교 한의과대학 내과학교실) ;
  • 정지천 (동국대학교 한의과대학 내과학교실)
  • Published : 1998.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was undertaken to determine whether Salviae-radix (SVR) extraction prevents the oxidant-induced cell injury and thereby exerts protective effect against oxidant-induced inhibition of tetraethylammonium uptake (TEA) in renal corticaJ sices. SVR (5%) attenuated $H_2O_2-induced$ inhibition of TEA uptake. $H_2O_2$ increased LDH release and lipid peroxidation in a dose-dependent manner. These changes were prevented by SVR extraction. The protective effect of SVR on LDH release was dose-dependent over the concentration range of 0.1-0.5%, and that on lipid peroxidation over the concentration ranges of 0.05-2%. SVR significantly prevented Hg-induced lipid peroxidation. SVR extraction (0.5%) increased cellular GSH content in normal and $H_2O_2-treated$ tissues. When slices were treated with 100 mM $H_2O_2$, catalase activity was decreased, which was prevented by 0.5% SVR extraction. The activity of glutathione peroxidase but not superoxide dismutase was significantly increased by 0.5% SVR extraction in $H_2O_2-treated$ tissuces. These results suggest that SVR has an antioxidant action and thereby exerts benefical effect against oxidant-induced impairment of membrane transport function. This effect of SVR is attributed to an increase in endogenous antioxidants such as GSH, catalase and glutathione peroxidase.

Keywords

References

  1. Kid lnt v.40 Reactive oxygen species and rat renal epithelial cells during hypoxia and reoxygenation Paller MS;Neurnann TV
  2. Lab lnvest v.51 Evidence for the role of oxygen free radicals in acute nephrotoxic nephritis Rehan A;Johnson KJ;Wiggins RC;Kunkel RG;Ward PA
  3. J Clin Invest v.81 Evidence of the role of hydroxyl radical in gentamicin induced acute renal failure in rats Walker PD;Shah SV
  4. Kid Int. v.39 The biology of ischemic renal injury Weinberg JM
  5. Kid lnt. v.43 Mechanisms of ischemic acute renal failure Bonventre JV
  6. Pharmacol Ther v.41 Biochemical modulation of cisplatin toxicity Borch RF;Markman M
  7. 中醫雜誌 no.6 急性腎功能衰竭的中醫臨床硏究 曹希和
  8. 實用中醫腎病學 張天;陳以平(主編)
  9. 동의신계학 두호경
  10. 實用中醫腎病學 張大寧
  11. 本草學 李尙仁
  12. 中藥大辭典 江蘇中醫學院(編)
  13. 本草從新 吳儀洛
  14. 中藥藥理與應用 王浴生(主編)
  15. 四川中醫 no.2 慢性腎衰中醫治則的探討 陳伯煊
  16. 江蘇中醫 no.1 老年慢性腎功能衰竭治療蠡言 劉燦康
  17. 陜西中醫 v.14 no.11 益腎通絡湯洽療慢性腎炎腎功能不全 46例 胡元奎;許建泰
  18. 福建中醫藥 v.23 no.4 健脾益腎活血祛瘀法治療慢性腎炎腎功能不全 張小華
  19. 中西醫結合雜誌 v.15 no.3 中醫藥硏究中有關自由基硏究近 許沛虎(外)
  20. 中成藥 v.15 no.11 丹參對老齡小鼠SOD和LPO的影響 郭忠興(外)
  21. 대한한방내과학회지 v.18 no.1 Oxidant에 의한 신장세뇨관 물질 이동계의 장애에 대한 丹參의 효과 金尙範;鄭智天
  22. Anal Biochem. v.86 Determination of malonaldehyde precusor in tissue by thiobarbituric acid test Uchiyama M;Mihara M
  23. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M.M.
  24. Methods Enzymol v.113 Determination of glutathione and glutathione disulfide in biological Samples Anderson ME
  25. Methods Enzymol. v.105 Catalase in vitro Aebi H
  26. Methods Enzymol v.5 Assays of glutathione peroxidase Flohe L;Gunzler WA
  27. Methods Enzymol v.105 Superoxide dismutase assays Flohe L;Otting F
  28. Renal tubular transport of organic cations Renal Transport of Organic Substances Rennick BR;Greger R;Lang F;Silbernagl S
  29. Lab. Invest. v.62 Biology of disease: Mechanisms of cell injury by activated oxygen species Farber, J. L.;Kyle, M. E.;Coleman, J. B.
  30. Am j Physiol. v.268 Role of lipid peroxidation in H2O2-induced renal epithelial (LLC- PKI) cell injury Salahudeen AK
  31. Kid lnt. v.50 Renal oxidant injury and oxidant response induced by mercury Nath KA;Croatt AJ;Likely S;Behrens TW;Warden D
  32. Membrane Lipid oxidation v.II antioxidant defence systems and oxidative Stress Ross D;Moldeus P;Vigo-Pelfrey C.
  33. J Lab Clin Med. v.119 Free radicals, antioxidants, and human disease:Where are we now? Halliwell B;Gutteridge JMC;Cross CE
  34. FASEB J v.9 Oxidative processes and antioxidative defense mechanisms in the againg brain Reiter RJ
  35. Annu Rev Biochem v.52 Glutathione Meister A;Anderson ME
  36. J Biol Chem v.260 Endogenous defence against the cytotoxicity of hydrogen peroxide in cultured rat hepatocytes Starke, PE;Farber JL