BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Structural and Dynamic Studies of the Central Segments in the Self-complementary Decamer DNA Duplexes d(ACGTATACGT)2 and d(ACGTTAACGT)2

  • Park, Jin-Young (Department of Chemistry, Korea Advanced Institute of Science and Technology) ;
  • Lee, Joon-Hwa (Department of Chemistry, Korea Advanced Institute of Science and Technology) ;
  • Choi, Byong-Seok (Department of Chemistry, Korea Advanced Institute of Science and Technology)
  • Published : 1998.01.31
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Abstract

The structures of the self-complementary decamer duplexes, $d(ACGTATACGT)_2$ (TATA-duplex) and $d(ACGTTAACGT)_2$, (TTAA-duplex) has been obtained in solution by proton NMR spectroscopy and restrained molecular dynamics. The duplexes are essentially B-type, with distortions apparent at the TATA and TTAA steps. Theses distortions and their effects on dynamics have been investigated by the measurement of imino proton exchange time of the base-pairs. The unusual opening kinetics of central A T base-pairs could be correlated to the abnormal structural properties of the corresponding sequences.

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