Optimization of Cellulase Production in Batch Fermentation by Trichoderma reesei

  • Yu, Xiao-Bin (School of Biotechnology, Wuxi University of Light Industry) ;
  • Nam, Joo-Heon (Department of Biological Engineering, Inha University epartment of Biological Engineering, Inha University epartment of Biological Engineering, Inha University) ;
  • Yun, Hyun-Shik (Department of Biological Engineering, Inha University epartment of Biological Engineering, Inha University epartment of Biological Engineering, Inha University) ;
  • Koo, Yoon-Mo (Department of Biological Engineering, Inha University epartment of Biological Engineering, Inha University epartment of Biological Engineering, Inha University)
  • Published : 1998.06.01

Abstract

Maximum cellulase production was sought by comparing the activities of the cellulases produced by different Trichoderma reesei strains and Aspergillus niger. Trichoderma reesei Rut-C30 showed higher cellulase activity than other Trichoderma reesei stains and Aspergillus niger that was isolated from soil. By optimizing the cultivation conditions during shake flask culture, higher cellulase production could be achieved. The FP(filter paper) activity of 3.7U/ml and CMCase (Carboxymethylcellulase) activity of 60U/ml were obtained from shake flask culture. When it was grown in 2.5L fermentor, where pH and DO levels are controlled, the enzyme activities were 133.35U/ml (CMCase) and 11.67U/ml(FP), respectively. Ammonium sulfate precipitation method was used to recover enzymes from fermentation broth. The dried cellulase powder showed 3074.9U/g of CMCase activity and 166.7U/g of FP activity with 83.5% CMCase recovery.

References

  1. Tappi J v.77 no.4 Comparison of enzyme-enhanced with conventional deinking of xerographic and laser-printed paper Jeffries, T. W.;J. H. Klungness;M. S. Sykes;K. R. Rutledge-Cropsey
  2. Tappi J v.77 no.10 Factors influencing enzyme deinking of recycled fiber Zeyer, C.;T. W. Joyce;J. A. Heitmann;J. W. Rucker
  3. Tappi J v.79 no.3 Industrial scale-up of enzyme-enhanced deinking of nonimpack printed toners Heise, O. U.;J. P. Unwin;J. H. Klungness;W. G. Fineran Jr.;M. Sykes;S. Abubak r
  4. J. Adv. Chem. Ser v.95 Cellulase production by Trichoderma reesei Mandels, M.;J. Weber
  5. Pure Appl. Chem v.59 Measurements of cellulase activities Ghose, T. K.
  6. Biotechnol. Bioeng v.23 Enhanced production of cellulae, hemicellulase and β-glucosidase by Trichoderma reesei Rut C-30 Tangnu, S.K.;H. W. Blanch
  7. Biotechnol. Bioeng v.21 Cellulase production and ammonia metabolism in Trichoderma reesei on high levels of cellulose Sternberg, D.;S. Dorval
  8. Dokl. Bolg. Akad. Nauk v.42 no.11 Biosynthesis of hydrolase enzymes from Trichoerma strain sp 94 Marinova, D.;A. Atev;K. Markov
  9. Appl. Microbiol. Biotechnol v.43 Effect of agitation on growth and enzyme production of Trichoderma reesei in batch fermentation Lejeune, R.;G. V. Baron
  10. Biotechnol. Lett v.12 Different temperature profiles of enzyme secretion by two common strains of Trichoderma reesei Merivuori, H
  11. Enzyme Microb. Technol v.2 Cellulases: biosynthesis and applications Ryu, D. D. Y.;M. Mandels
  12. Can. J. Microbiol v.23 β-glucosidase microbial production and effect on enzymatic hydrolysis of cellulose Sternberg, D.;P. Vijayakumar;E. T. Reese