KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Effect of pH and Temperature on the Production of Biosurfactant by Pseudomonas aeruginosa YPJ-80 and Its Separation

Pseudomonas aeruginosa YPJ-80에 의한 생물계면활성제 생산에 미치는 pH 및 온도의 영향과 생물계면활성제의 분리

  • 박창호 (경희대학교 화학공학과 및 환경연구소) ;
  • 손창규 (경희대학교 화학공학과 및 환경연구소) ;
  • 김성훈 (경희대학교 화학공학과 및 환경연구소) ;
  • 안도균 (경희대학교 화학공학과 및 환경연구소)
  • Published : 1998.10.01
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Abstract

Temperature and pH conditions were studied for an effective biosurfactant production by Pseudomonas aeruginosa YPJ-80. Efficient methods of biosurfactant separation were also investigated. pH-uncontrolled experiments at 35$^{\circ}C$ and an initial pH of 8 resulted in the best cell growth (3.6 g/L) and biosurfactant production (0.073 g biosurfactant/g cell). Biosurfactant separation was most efficient using solvent extraction with chloroform/methanol (2:1 vol%) followed by acidification using 1N HCl.

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