KSBB Journal
- Volume 13 Issue 5
- /
- Pages.535-539
- /
- 1998
- /
- 1225-7117(pISSN)
- /
- 2288-8268(eISSN)
Purification and Characterization of Recombinant Monellin Produced from Yeast Culture Medium
효모 배양액으로부터 재조합 모넬린의 정제와 특성 연구
Abstract
The monellin, a sweet-taste protein, was expressed and secreted in Saccharomyces cerevisiae. The secreted menellin was concentrated using an ultrafiltration membrane with a nominal molecular weight cut off of 3,000 or by ammonium sulfate precipitation. The monellin was purified by G-25 gel filtration chromatography, followed by CM-Sepharose ion exchange chromatography. The purified monellin was characterized by SDS-PAGE (SDS-Polyacrylamide Gel Electrophoresis) and PHLC. The molecular weight of monellin was found to be 10,700 dalton, and its purity was over 95%.