Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Effect of Anti-Phospholipid Antibodies and Phospholipids on In Vitro Fertilization and In Vitro Development of Mouse Oocytes

항인지질 자가항체 및 각종 인지질의 처리가 Mouse 난자의 체외수정 및 초기 배발생에 미치는 영향

  • Ko, J. J. (College of Medicine, Pochon CHA University, Infertility Medical Center of CHA General Hospital) ;
  • Chung, H. M. (College of Medicine, Pochon CHA University, Infertility Medical Center of CHA General Hospital) ;
  • Shim, S. W. (College of Medicine, Pochon CHA University, Infertility Medical Center of CHA General Hospital) ;
  • Kim, N. K. (College of Medicine, Pochon CHA University, Infertility Medical Center of CHA General Hospital) ;
  • Lim, J. M. (College of Medicine, Pochon CHA University, Infertility Medical Center of CHA General Hospital) ;
  • Lee, H. K. (College of Medicine, Pochon CHA University, Infertility Medical Center of CHA General Hospital) ;
  • Park, C. (College of Medicine, Pochon CHA University, Infertility Medical Center of CHA General Hospital) ;
  • Kim, S. Y. (College of Medicine, Pochon CHA University, Infertility Medical Center of CHA General Hospital) ;
  • Cha, K. Y. (College of Medicine, Pochon CHA University, Infertility Medical Center of CHA General Hospital)
  • 고정재 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 정형민 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 심상우 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 김남근 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 임정묵 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 이혜경 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 박찬 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 김시영 (포천중문의과대학교 차병원 여성의학연구소) ;
  • 차광열 (포천중문의과대학교 차병원 여성의학연구소)
  • Published : 1998.08.01
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Abstract

Anti-phospholipid antihodies (aPL) have important roles in various pregnancy complications such as recurrent miscarrige, growth retardation, placental abruption and stillbirth. However, their biological actions on preimplantation development of oocytes are still unclear. In this study, we investigated whether either aPL containing sera or phospholipids could affect in vitro fertilization and development of mouse oocytes. Sera used in this study were collected from three patients and the presence of aPL in the sera was confirmed by enzymatic-linked immunosorbent assay. When mouse oocytes were cultured in a serum-free, Chatot, Ziomek and Bavister (CZB) medium (Experiment 1), addition of aPL-containing sera (10%) to CZB medium did not. significantly (P>0.05) influence sperm penetration of oocytes. However, development to the blastocyst stage was significantly (P<0.05) inhibited by serum addition, and formation of morulae (16-23% vs. 58%) and blastocysts (0-4% vs. 21%) was markedly reduced compared with no addition (Experiment 2). In Experiment 3, pronuclear stage embryos were cultured for 96 h in GZB medium supplemented with 1 $\mu$g /ml phosphatidyl ethanolamine, 1 $\mu$g/ml phosphatidyl inositol or 1 $\mu$g /ml phosphatidyl choline. No increase in embryo development was found after addition of the phospholipids to CZB medium. These results suggest that 1) aPL have an inhibitory role in preimplantation development of mouse embryos, and that 2) the action of aPL may be related to a specific phospholid (s) rather than the tested phospholipids in the present study.

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