RAPD 및 약제저항성을 이용한 감귤 검은점무늬병균의 유전적 다양성 분석

Genetic Diversity of Phomopsis citri with Random Amplified Polymorphic DNA (RAPD) and Fungicide Resistance

  • 고영진 (순천대학교 응용생물원예학부) ;
  • 서정규 (순천대학교 응용생물원예학부) ;
  • 이태선 (순천대학교 응용생물원예학부) ;
  • 송장훈 (농촌진흥청 제주감귤연구소) ;
  • 권혁모 (농촌진흥청 제주감귤연구소) ;
  • 문덕영 (농촌진흥청 제주감귤연구소) ;
  • 문두길 (제주대학교 원예생명과학부) ;
  • 한해룡 (제주대학교 원예생명과학부)
  • 발행 : 1998.04.01

초록

Gentic diversity of 42 isolates of Phomopsis citri was analyzed with random amplified polymorphic DNA(RAPD) and fungicide resistance. RAPD profiles of genomic DNA of the isolates of P. citri and the degrees of their resistance to the fungicides mancozeb and propineb suggested the occurrence of genetic differentiation of P. citri distributed in Cheju. The isolates showed genetically diverse RAPD profiles according to the host species collected even from the same collection site and also according to the geographic origin collected even from the same host species. High levels of resistance to fungicides mancozeb and propineb were observed among the isolates of P. citri. However, there was no correlation between RAPD profiles of genomic DNA and levels of fungicide resistance of the isolates, suggesting that fungicide resistance of P. citri occurred irrespective of the host and geographic origin.

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