Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Enzymatic Characterization of Peroxidase from Soybean Sprouts

콩나물 Peroxidase의 효소적 특성

  • 박인식 (동아대학교 식품영양학과) ;
  • 이민경 (동아대학교 식품영양학과)
  • Published : 1998.12.01
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Abstract

Enzymatic characterization of peroxidase(E.C. 1.11.1.7) from soybean sprouts was investigated. The optimum pH of the purified peroxidase was 7.0 and relatively stable at pH 6.0~7.0. And the optimum temperature was 50oC. The enzyme was most active with guaiacol as a substrate, followed by (＋)catechin, pyrogallol and p phenylenediamine. The Km values for guaiacol and H2O2 were 4.2mM and 2.5mM, respectively. L Ascorbic acid and 2 mercaptoethanol greatly inhibited the enzyme activity, while Cu2+, Co2+ and Ni2+ activated the enzyme.

Keywords

References

  1. Food Technology v.40 Blanching of vegetables for freezing which indicator enzyme to choose Williams, D. C.;Lim, M. H.;Chen, A. O.;Pangborn, R. M.;Whitaker, J. R.
  2. Hort. Science v.24 Quantification of net enzymatic activity in developing peach fruit using computer video image analysis Stutle, G. W.
  3. J. Food Sci. v.60 Peroxidase stability and reactivation after heat treatment and manothermosonication Lopez, P.;Burgos, J.
  4. J. Korean Soc. Food Nutr. v.19 Factors affecting thermal inactivation and reactivation of Korean-radish peroxidase Lee, K. A.;Hong, J. M.;Kim, G. N.;Park, I. S.
  5. Korean Biochem. J. v.21 Purification and characterization of anionic isoperoxidase from Korean-radish root Yoo, W.;Kim, S.
  6. Agric. Biol. Chem. v.35 Properties and structure of peroxidase isozymes of Japanese-radish Morita, Y.;Yoshida, C.;Maeda, Y.
  7. Korean J. Food Sci. Technol. v.25 Isolation and characterization of peroxidase from Jerusalem artichoke tubes Yoon, E.;Kang, S.;Noh, B.;Choi, E.
  8. J. Agric. Food Chem. v.29 Soybean peroxidases : purification and some properties Sessa, D. J.;Anderson, R. L.
  9. J. Food Sci. v.46 Some biochemical properties of soluble and bound potato tuber peroxidase Kahn, V.;Goldshmidt, S.;Amir, J.;Granit, R.
  10. J. Food Sci. v.48 Characterization of soluble and bound peroxidases in green asparagus Wang, Z.;Luh, B.
  11. J. Plant Physiol. v.135 On substrate specificity of peroxidases involved in the lignification process Pang, A.;Catesson, A.;Francesh, C.;Rolando, C.;Goldberg, R.
  12. Biochem. Biophys. Res. Communs. v.163 Immunological similarity of the cationic and the anionic peanut peroxidase isozymes Hu, C.;van Huystee, R. B.
  13. Biochem. J. v.163 Generation of hydrogen peroxidase, superoxide and hydroxyl radicals during the oxidation of dihydroxyfumaric acid by peroxidase Halliwell, B.
  14. Biochemistry v.21 Oxidation of indole-3-acetic acid by peroxidase : Involvement of reduced peroxidase and compound Ⅲ with superoxide as a product Smith, A. M.;Morrison, W. L.;Milham, P. J.
  15. Plant Physiol. v.75 Characterization of peroxidase in plant cells Chibbar, R. N.;van Huystee, R. B.
  16. Photochem. Photobiol. v.28 Superoxide and peroxidase-catalysed reactions. Oxidation of dihydroxyfuromate, NADH and dithiothreitol by horseradish peroxidase Halliwell, B.;Rycker, J. de
  17. J. Plant. Physiol. v.129 Catabolites of chlorophyll in senescent leaves Matile, P.;Ginsburg, S.;Schellenberg, M.;Thomas, H.
  18. Plant Physiol. v.87 Induction of 33-kD and 60-kD peroxidases during ethylene-induced senescence of cucumber cotyledons Abeles, F. B.;Dunn, L. J.;Morgens, P.;Callahan, A.;Dinterman, R. E.;Schmidt, J.
  19. J. Biochem. v.92 Isolation and characterization of five neutral isoenzymes of horse-radish peroxidase Aibara, S.;Yamashita, H.;Mori, E.;Kato, M.;Morita, Y.
  20. Food Chem. v.46 Purification of an anionic peroxidase isoenzyme from mango Khan, A. A.;Robinson, D. S.
  21. Eur. J. Biochem. v.151 Plant peroxidases. Their primary, secondary and tertiary structures, and relation to cytochrome c peroxidase Welinder, K. G.
  22. Korean J. Food Sci. Technol. v.19 Purification and properties of the peroxidase in castanea semen Oh, S. H.;Kim, Y. H.;Lee, S. N.
  23. J. Korean Soc. Food Nutr. v.21 Purification and some properties of peroxidase from the fruit Malus sieboldii rehder Yang, H. C.;Son, H. S.;Shim, K. K.;Oh, C. H.;Choi, D. S.
  24. Korean J. Food Sci. Technol. v.23 Isolation and characterization of fuji apple peroxidase Jee, W. J.;Cho, N. S.;Kim, I. C.;Park, K. H.;Choi, E. H.
  25. J. Food Sci. v.41 Peroxidase reaction and orange juice quality Bruemmer, J. H.;Roe, B.;Bowen, E. R.
  26. CRC Crit. Rev. Food Sci. Nutri. Sep. v.15 Polyphenol oxidase and peroxidase in fruit and vegetables Vamos-Vigyazo, L.