약학회지 (YAKHAK HOEJI)

  • 제42권1호
  • /
  • Pages.53-58
  • /
  • 1998
  • /
  • 0377-9556(pISSN)
  • /
  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

해모필루스 K-12가 생산하는 황산전이효소의 고정화

Immobilization of Arylsulfate Sulfotransferase Obtained from Haemophilus K-12

  • 발행 : 1998.02.01
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초록

A novel type of sulfotransferase, arylsulfate sulfotransferase (EC 2.8.2.22) purified from Haemophilus K-12, an intestinal bacterium of a mouse, was immobilized onto AH-S epharose 4B, CH-Sepharose 4B and DEAE-celluose. The enzyme was stabilized for storage more markedly by covalent immobilization onto AH-Spharose 4B or CH-Sepharose 4B and by adsorptive immobilization onto DEAE-cellulose than the free enzyme. The optimal pH and acceptor substrate specificity of immobuized enzyme were similar to those of the free enzyme.

키워드

참고문헌

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