Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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The Determination of 5-Fluorourasil in Human Plasma by a Gas Chromatography-Mass Spectrometry

GC-MS에 의한 혈중 5-fluorouracil의 정량법

  • Shin, Ho-Sang (Department of Environmental Education, Kongju National University) ;
  • Seo, Bae-Seck (Department of Chemistry, Catholic University Medical College) ;
  • Oh, Yun-Suk (Korea Environment & Water Works Institute) ;
  • Park, Sung-Woo (National Institute of Scientific Investigation)
  • 신호상 (공주대학교 환경교육과) ;
  • 서배석 (가톨릭대학교 의과대학 화학과) ;
  • 오윤숙 (한국환경수도연구소) ;
  • 박성우 (국립과학수사연구소)
  • Received : 1997.08.09
  • Published : 1998.02.01
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Abstract

A gas chromatography-mass spectrometry method for the determination of 5-fluorourasil in human plasma is described. The method involves a single extraction procedure with 10 ml of isopropanol-ether(20:80) solution and pentafluoro-benzylation. Samples were injected using an automatic injector, followed by separation on a nonpolar capillary column and detection with a mass selective detector(MSD). No endogeneous compounds were found to interfere. The detection limit, based upon an assayed plasma volume of 0.5, was 3 ng/ml. The extraction yield was found to be above 80%. Plasma 5-FU concentrations were determined by this method in about 500 plasma samples from cancer patients undergoing treatment with 5-FU. This method is suitable for monitoring of 5-FU in plasma of cancer patients.

GC-MS를 이용한 사람의 혈중 5-fluorouracil(5-FU)의 정량법이 연구되었다. 5-FU은 isopropanol-ether(20:80) 혼합용매 10ml로서 추출하였고 pentafluoro-benzylation에 의해 유도체화 하였다. 시료주입은 자동으로 수행하였으며 분리에 사용한 칼럼은 비극성 capillary column이었고 검출은 MSD로 하였다. 위의 조건하에서 내인성 물질들의 방해현상은 없었고 검출한계는 0.5 ml의 혈액시료를 사용하였을 때 3 ng/ml이었으며 추출효율은 80%이상을 나타냈다. 5-FU로 항암치료를 받는 환자의 시료 500개중에 혈중 5-FU의 농도 측정에 적용하였으며 이 방법이 우수한 monitoring법임을 알 수 있었다.

Keywords

Acknowledgement

Supported by : 과학기술처