Insulin-like growth factor-I(IGE-I)이 조피볼락의 체중 및 혈액중 IGF binding proteins에 미치는 영향

Effects of Insulin-like Growth Factor-I (IGF-I) on Body Weight and the Cocentration of Serum IGF Binding Proteins in Korean Rockfish (Sebastes schlegeli)

동물성장인자인 insulin-like growth factor-I (IGF-I)이 조피볼락의 성장 및 혈액중에 존재하는 IGF binding proteins (IGFBPs)에 미치는 영향을 검토하기 위하여 human recombinant IGF-I을 투여하여 분석한 결과, 절식중의 조피볼락 체중은 IGF-I 투여군이 생리식염수만 투여한 대조군 보다 체중 증가효과가 있었으며, 혈액중 glucose농도는 IGF-I의 투여군이 혈당저하효과를 나타내었다. 그리고 혈액중 IGFBP-3는 IGF-I투여군에서 증가하였으며, IGFBP-1은 감소하였다.

The effect of insulin-like growth factor-I (IGF-I) on circulating insulin-like growth factor binding proteins (IGFBPs) in the Korean rockfish, Sebastes schlegeli, was assessed after injected of recombinant human IGF-I (6 $\mu$g/100 g body weight). Growth and metabolic status of each fish were assessed by determing body length and body weight changes, and serum glucose concentration. Serum IGF binding proteins concentrations were assessed by the Western ligand blot procedure using $^{125}I$-labeled human IGF-I tracer. The fish received IGF-I were Heavier than the saline-injected control fish after 2 weeks of treatment. Plasma IGFBP-3 concentration inclosed, but plasma IGFBP-1 and glucose levels decreased significantly after administration. Taken together, the findings of this study suggest that human IGF-I is biologically active in Korean rockfish and may be of significance in metabolic and growth-related processes.