Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Development of an enzyme immunoassay for detection of Escherichia coli O157 in meat

식육중 Escherichia coli O157 검출을 위한 enzyme immunoassay 기법 개발

  • Jung, Byeong-yeal (Department of Bacteriology, National Veterinary Research and Quarantine Services, MAF) ;
  • Jung, Suk-chan (Department of Bacteriology, National Veterinary Research and Quarantine Services, MAF) ;
  • Cho, Dong-hee (Department of Bacteriology, National Veterinary Research and Quarantine Services, MAF) ;
  • Kim, Jong-yeom (Department of Bacteriology, National Veterinary Research and Quarantine Services, MAF) ;
  • Park, Yong-ho (College of veterinary Medicine, Seoul National University) ;
  • Shin, Sang-jae (New York State College of Veterinary Medicine, Cornell University) ;
  • Kim, Sung-guk (New York State College of Veterinary Medicine, Cornell University) ;
  • Kim, Bong-hwan (College of Veterinary Medicine, Kyungpook National University)
  • 정병열 (농림부 수의과학검역원 세균과) ;
  • 정석찬 (농림부 수의과학검역원 세균과) ;
  • 조동희 (농림부 수의과학검역원 세균과) ;
  • 김종염 (농림부 수의과학검역원 세균과) ;
  • 박용호 (서울대학교 수의과대학) ;
  • 신쌍재 (코넬대학교 수의과대학) ;
  • 김성국 (코넬대학교 수의과대학) ;
  • 김봉환 (경북대학교 수의과대학)
  • Received : 1998.03.25
  • Published : 1998.12.22
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Abstract

A sensitive and rapid enzyme immunoassay(EIA) to detect Escherichia coli O157 in ground beef was developed by using a sandwich type assay with polyclonal antibodies to E coli O157. E coli O157 in ground beef could be detected within 15hr, including incubation for 12hr in enrichment broth and 3hr in immunoassay. The EIA could detect $1.3{\times}10^5$ cells of E coli O157/g of ground beef without enrichment. The lowest limit of detection was 0.23 E coli O157 per g of meat after enrichment. Confirmation was required in the positive specimens in the EIA by culture method even though the negative specimens were not. These results suggested that the immunoassay could be a very efficient method for the screening E coli O157 in meat.

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