BMB Reports
- Volume 31 Issue 4
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- Pages.377-383
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- 1998
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- 1976-670X(eISSN)
Thioltransferase (Glutaredoxin) from Chinese Cabbage: Purification and Properties
- Cho, Young-Wook (Division of Life Sciences, Kangwon National University) ;
- Park, Eun-Hee (College of Pharmacy, Sookmyung Women's University) ;
- Lim, Chang-Jin (Division of Life Sciences, Kangwon National University)
- Received : 1998.03.23
- Published : 1998.07.31
Abstract
Thioltransferase, also known as glutaredoxin, was purified from Chinese cabbage (Brassica campestris ssp. napus var. pekinensis) by a combination of ion-exchange chromatography and gel filtration. Its purity was confirmed by SDS-polyacrylamide gel electrophoresis and its molecular weight was estimated to be about 12,000 which is comparable with those of most known thioltransferases. The enzyme utilizes 2-hydroxyethyl disulfide, S-sulfocysteine,
Keywords
- Chinese cabbage (Brassica campestris ssp. napus var. pekinensis);
- Glutaredoxin;
- Purification;
- Thioltransferase