한국균학회지 (The Korean Journal of Mycology)

한국균학회 (The Korean Society of Mycology)
권호 표지

ITS II 영역의 DNA 염기서열 분석에 의한 불로초(Ganoderma)속의 계통분류학적 고찰

Phylogenetic Study of Ganoderma spp. Based on the DNA Sequences in ITS II Region

  • 박동석 (농업과학기술원 분자유전과) ;
  • 고승주 (농업과학기술원 분자유전과) ;
  • 류진창 (농업과학기술원 분자유전과) ;
  • 성재모 (강원대학교 농생물학과)
  • Park, Dong-Suk (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Go, Seung-Joo (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Ryu, Jin-Chang (Division of Molecular Genetics, National Institute of Agricultural Science and Technology) ;
  • Sung, Jae-Mo (Department of Agricultural Biology, Kangwon National University)
  • 발행 : 1999.02.28
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초록

불로초의 계통 분류학적 조사를 위해 불로초속 중 8종(Ganoderma lucidum, G. tsugae, G. pfeifferi, G. resinaceum, G. australe-applanatum, G. oregonense, G. neo-japonicum, G. applanatum) 12균주와 out-group 균주로는 Inonotus xeranticus의 rDNA ITS II를 PCR로 증폭하여 염기서열을 비교 조사하였다. 불로초 속 중 ITS II 영역의 길이는 $247{\sim}257\;bp$로 분포하였고 종간 상동성은$70{\sim}100%$ 로 조사되었다. 계통도를 분석한 결과는 5개의 군(cluster)을 형성하였고 G. tsugae는 각각의 G. lucidum과 하나의 clade를 이루어 두 종은 서로 매우 밀접하게 진화된 것으로 사료되었다. 본 조사에서는 국내에서 재배되고 있는 불로초(G. lucidum)는 그 발생지 및 국외 ITS염기서얼 자료와 비교 및 분석할 때 G. tsugae로 하는 것이 타당할 것으로 사료되었다.

The internal transcribed spacer II regions (ITS II) of the ribosomal DNA gene repeat from Ganoderma spp. were amplified using polymerase chain reaction (PCR) and sequenced. Sequences from 9 species including Ganoderma lucidum, G. tsugae, G. pfeifferi, G. resinaceum, G. australe-applanatum, G. oregonense, G. neo-japonicum, G. applanatum and Inonotus xeranticus as an out-group were compared. The spacer regions of them were $247{\sim}257$ nucleotides in length and contained partial sequences of 5.8S and 25S gene. The reciprocal homologies of each ITS II sequence of the species were in the range of $70{\sim}100%$ except outgroup species, I. xeranticus. According to the analysis of ITS II sequences, Ganoderma spp. constructed 5 clusters. Ganoderma lucidum isolates were to be divided into two groups. One group was consisted of isolates from South Korea. The other group comprised isolates from UK. G. lucidum isolates belonging to the group I were closely related with G. tsugae. These results suggested that G. lucidum from Korea should be G. tsugae, otherwise G. tsugae was to be synonym of G. lucidum.

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