The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Cloning of a Gene Specifically Expressed During Early Stage of Fruiting Body Formation in Flammulina velutipes

팽이버섯의 자실체형성 초기과정에서 특이적으로 발현하는 유전자의 클로닝

  • Kim, Dool-Yi (Laboratory of Forest Chemistry, Department of Forest Science, Faculty of Agriculture, Hokkaido University) ;
  • Azuma, Tomo-Nori (Hokkaido Forest Products Research Institute)
  • 김둘이 (북해도대학 농학부 삼림과학과) ;
  • 동지칙 (일본 북해도립 임산시험장)
  • Published : 1999.06.30
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Abstract

A cDNA library was constructed using mRNA from the cells of 7-day-old cultures of Flammulina velutipes after induction of fruiting treatment. A cDNA clone, FVFD16 (Flammulina velutipes fruiting body differentiation), was selected by differential screening. The expression property of the FVFD16 gene was examined by Northern blot analysis. FVFD16 represents mRNA that is specifically expressed during differentiation of fruit bodies. The conspicuous accumulation of the FVFD16 mRNA was detected in 4-day-old and 1-day-old cultures. The nucleotide sequence of the FVFD16 gene was determined and the mRNA contained an open reading frame that encoded a putative protein of 128 amino acid residues (13.5 kDa).

팽이버섯의 자실체 분화 과정에서 특이적으로 발현하는 유전자 분리를 위한 cDNA library는 발이처리 후 7일째 배양한 균사체의 mRNA에 의해 만들어졌다. cDNA클론 FVFD16(Flammulina velutipes fruiting body differentiation)은 자실체 분화 과정에서 특이적으로 발현되는 클론으로 differential screening에 의해 선발되었다. Northern 분석에 의해 FVFD16의 발현 특성을 관찰한 결과, 1일과 4일째의 균사체에서 현저한 발현량을 나타내었다. FVFD16의 염기 서열을 검색한 결과, FVFD16의 mRNA는 open reading frame을 포함한 128의 아미노산 잔기(13.5kDa)를 가진 단백질로 추정되었다.

Keywords

References

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