Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Isolation of 3,4-Dihydroxybenzoic Acid, Which Exhibits Antimicrobial Activity, from Fruits of Gardenia jasminoides Ellis

치자 열매에서 항미생물 활성을 갖는 3,4-Dihydroxybenzoic Acid의 분리

  • Yim, Cheol-Keun (Department of Food Science and Technology, Chonnam National University) ;
  • Moon, Jae-Hak (Department of Nutrition, University of Tokushima) ;
  • Park, Keun-Hyung (Department of Food Science and Technology, Chonnam National University)
  • 임철근 (전남대학교 식품공학과) ;
  • 문제학 (일본 덕도대학 의학부 영양학과) ;
  • 박근형 (전남대학교 식품공학과)
  • Published : 1999.10.31
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Abstract

The methanol extract of Gardenia jasminoides Ellis showed antimicrobial activity against bacteria and yeasts. The extract was successively purified with solvent fractionation, silica gel adsorption column chromatography, Sephadex LH-20 column chromatography, octadecylsilane column chromatography. The purified active substance was isolated by high performance liquid chromatography. The isolated compound was 3,4-dihydroxybenzoic acid which was determined by mass spectrometer, gas chromatograph-mass spectrometer, $^{1}H-nuclear$ magnetic resonance, $^{13}C-nuclear$ magnetic resonance and two-dimensional nuclear magnetic resonance. The content of 3,4-dihydroxybenzoic acid was $32.7\;{\mu}g/g$ in dried fruits of Gardenia jasminoides Ellis.

치자(Gardenia jasminoides Ellis) 열매의 methanol추출물은 Gram양성 세균, Gram음성 세균, 효모 등의 미생물에 대하여 항미생물 활성을 보여, solvent fractionation, silica gel adsorption column chromatography, Sephadex LH-20 column chromatography, ODS column chromatography, HPLC등에 의해 얻어진 활성 물질을 MS, GC-MS, $^{1}H-NMR,\;^{13}C-NMR$ 그리고 2D-NMR 등의 기기 분석에 의해 3,4-dihydroxybenzoic acid는 동정하였다. 치자 열매에서 3,4-dihydroxybenzoic acid가 항미생물 활성물질로 분리 동정된 것은 처음으로 생각된다. 3,4-dihydroxybenzoic acid는 건조 치자열매에 g당 $32.7\;{\mu}g$ 정도 함유되어 있었다.

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