Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Isolation and Identification of Fibrinolytic Enzyme Producing Strain from Shrimp Jeot-Gal, a Tiny Salted Shrimps, and Medium Optimization for Enzyme Production

새우젓에서 혈전용해효소 생산균주의 분리, 동정 및 효소생산 배지의 최적화

  • Jang, Sun-Ae (Department of Food and Nutrition, Chungnam National University) ;
  • Kim, Myung-Hee (Environmental Bioresources Lab., Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Myung-Sun (Department of Food and Nutrition, Chungnam National University) ;
  • Lee, Myung-Ja (Department of Food and Nutrition, Chungnam National University) ;
  • Jhee, Ok-Hwa (Department of Food and Nutrition, Chungnam National University) ;
  • Oh, Tae-Kwang (Environmental Bioresources Lab., Korea Research Institute of Bioscience and Biotechnology) ;
  • Sohn, Cheon-Bae (Department of Food and Nutrition, Chungnam National University)
  • 장순애 (충남대학교 식품영양학과) ;
  • 김명희 (생명공학연구소 환경생물소재연구실) ;
  • 이명선 (충남대학교 식품영양학과) ;
  • 이명자 (충남대학교 식품영양학과) ;
  • 지옥화 (충남대학교 식품영양학과) ;
  • 오태광 (생명공학연구소 환경생물소재연구실) ;
  • 손천배 (충남대학교 식품영양학과)
  • Published : 1999.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

A strain of potential producer of fibrinolytic enzyme was isolated from shrimp Jeot-Gal, a tiny salted shrimps, and identified as Bacillus sp.. The preliminary experiment showed an enzyme yield of 18 U/mL in medium for screening. The carbon, nitrogen and salts significantly influenced the fibrinolytic enzyme production. An optimized medium containing 2% skim milk, 2% soluble starch and 3% NaCl (pH 7.5) after 72 hrs fermentation time at $37^{\circ}C$ yielded 3-fold increase in enzyme production, 62 U/mL.

새우젓으로부터 혈전용해효소를 생산하는 균주를 분리하여 Bacillus sp.로 동정하였다. Bacillus sp. S19는 screening 배지에서는 18 U/mL의 효소를 생산하였다. 그러나 2% soluble starch, 2% skim milk, 3% NaCl(pH 7.5)의 최적화된 배지로 $37^{\circ}C$에서 72시간 동안 배양하였을 경우 효소활성이 약 3배 증가된 62 U/mL의 효소 생산을 결과하였다.

Keywords