호도약침액(胡桃藥鍼液)이 가토(家兎) 뇌조직(腦組織)의 Na+-pump 활성(活性) 장애(障碍)에 미치는 영향(影響)

The Effects of t-butylhydroperoxide (tBHP) and Juglandis Semen on Brain Na+-Pump Activity

  • 김동훈 (동의대학교 한의과대학 침구경혈학교실) ;
  • 장경전 (동의대학교 한의과대학 침구경혈학교실) ;
  • 송춘호 (동의대학교 한의과대학 침구경혈학교실) ;
  • 안창범 (동의대학교 한의과대학 침구경혈학교실)
  • Kim, Dong-Hoon (Dept. of Acu. & Mox., College of Oriental Medicine, Dong Eui UNIV) ;
  • Jang, Kyung-Jeon (Dept. of Acu. & Mox., College of Oriental Medicine, Dong Eui UNIV) ;
  • Song, Choon-Ho (Dept. of Acu. & Mox., College of Oriental Medicine, Dong Eui UNIV) ;
  • Ahn, Chang-Beohm (Dept. of Acu. & Mox., College of Oriental Medicine, Dong Eui UNIV)
  • 발행 : 1999.02.28


This study was undertaken to determine whether Juglandis Semen (JAS) extraction exerts protective effect against oxidant-induced inhibition of $Na^+$-pump activity in cerebral cortex. $Na^+$-pump activity was estimated by measuring ouabain-sensitive oxygen consumption. The oxygen consumption significantly inhibited by 1 mM t-butylhydroperoxide (tBHP), which was prevented by addition of 2% JAS extraction. The oxygen consumption was increased by an increase in $Na^+$ concentration from 5 to 100mM, $K^+$ concentration from 0.5 to 10 mM, and $Mg^{++}$ concentration from 0.2 to 5 mM. These changes in ion concentrations did not affect the inhibitory effct of tBHP and protective action of JAS on oxygen consumption. tBHP(1mM) produced a significant increase in lipid peroxidation in cerebral cortex, which was prevented by 2% JAS extraction. These result suggest that JAS exerts protective effect against tBHP-induced inhibition of $Na^+$-pump activity in the cerebral cortex, this effect may be due to by an antioxidant action.



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