YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effect of Pini Folium Extract on AP-1 (c-fos/c-jun) in Cancer Cells

암세포에서 송엽의 AP-l (c-fos/c-jun)에 미치는 영향

  • 박건구 (아산생명과학연구소 분자유전연구실) ;
  • 장혜숙 (울산대학교 의과대학) ;
  • 이정교 (울산대학교 의과대학) ;
  • 최승훈 (경희대학교 한의과대학)
  • Published : 1999.02.01
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Abstract

Phorbol ester, growth factors activities are mediated by unclear transcription factors, the c-Fos and c-Jun, which can regulate transcriptional activation through specific DNA sites and by forming the transcription factor AP-l, which usually mediates cell proliferation and differentiation signals. We explored effects of Pini Folium extract (API-l) on AP-l activity. Western blot analysis confirmed that API-l decreased levels of c-Fos or c-Jun protein induced by the tumor promoter Phorbol 12-myristate 13-acetate (PMA; 200 nM). Transient transfection assays with a c-fos promoter reporter construct showed that API-l decreased transcription activity by ore than 50~60%. However, treatment of API-l activity studied further. The main substances were fractionated into dichloromethane layer. Futhermore, API-l extract repressed the [$^3H$]-thymidine uptake in C6 glioma cells, indicating that this extract could be included in a new type of modulator in the mitogenesis.

Keywords

References

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