Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Antioxidant Flavonoids and Chlorogenic Acid from the Leaves of Erobotrya japonica

  • Jung, Hyun-Ah (Department of Food ad Life Science, Pukyung National University) ;
  • Park, Jong-Cheol (Department of Oriental Medicine Resoureces, Sunchon Naitonal University) ;
  • Chung, Hae-Young (Department of Pharmacy, Pusan National University) ;
  • Kim, Jong (Masan College) ;
  • Choi, Jae-Sue (Department of Food ad Life Science, Pukyung National University)
  • Published : 1999.04.01
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Abstract

The antioxidant activity of Eriobotrya japonica was determined by measuring the radical scavenging effect on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and lipid peroxidation produced when mouse liver homogenate was exposed to the air at $37^{\circ}C$, using 2-thiobarbituric acid (TBA). The methanol extract and its factions of Eriobotrya japonica leaves showed strong antioxidant activity. The antioxidant activity of EtOAc and n-BuOH soluble fractions were stronger than the others, and were further purified by repeated silica gel, MCl gel CHP-20P, and Sephadex LH-20 column chromatography. Antioxidant chlorogenic acid, quercetin-3-sambubioside from n-BuOH fraction and methyl chlorogenate, kaempferol- and quercetin-3-rhamnosides, together with the inactive ursolic acid and$ 2{\alpha}$-hydroxyursolic acid from EtOAc fraction were isolated. Antioxidant flavonoids and chlorogenic acid also showed prominent inhibitory activity against free radical generation in dichlorofluorescein (DCF) method.

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